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1) Determine the MICS on the simulated microbroth-dilution results from an MIC Assay on Staphylococcus aureus.

1) Determine the MICS on the simulated microbroth-dilution results from an MIC Assay on Staphylococcus aureus.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1) Determine the MICS on the simulated microbroth-dilution results from an MIC Assay on Staphylococcus aureus. Legends: PC: Positive Control (No antimicrobial agent) NC: Negative Control (No bacteria) No Growth With Growth µg/mL: 128 64 32 16 8 4 2 1 0.5 0.25 PC NC 2 3 4 5 7 8 9 10 11 12 Amoxicillin A Ticarcillin Piperacillin C D Cefotaxime E Ertapenem F Nalidixic Acid G Ciprofloxacin H Gentamicin а. Fill out the MICS on the table below. MIC Susceptibility to Staphylococcus aureus Antimicrobial Agent (Hg/mL) (Susceptible, Intermediate, or Resistant) Amoxicillin Ticarcillin Piperacillin Cefotaxime Ertapenem Nalidixic Acid Ciprofloxacin Gentamicin B
Transcribed Image Text:1) Determine the MICS on the simulated microbroth-dilution results from an MIC Assay on Staphylococcus aureus. Legends: PC: Positive Control (No antimicrobial agent) NC: Negative Control (No bacteria) No Growth With Growth µg/mL: 128 64 32 16 8 4 2 1 0.5 0.25 PC NC 2 3 4 5 7 8 9 10 11 12 Amoxicillin A Ticarcillin Piperacillin C D Cefotaxime E Ertapenem F Nalidixic Acid G Ciprofloxacin H Gentamicin а. Fill out the MICS on the table below. MIC Susceptibility to Staphylococcus aureus Antimicrobial Agent (Hg/mL) (Susceptible, Intermediate, or Resistant) Amoxicillin Ticarcillin Piperacillin Cefotaxime Ertapenem Nalidixic Acid Ciprofloxacin Gentamicin B
b. Compare the MIC above with the MIC Breakpoints on the Sample Standards below and fill out the Susceptibility Result column on the table above. Sample Performance Standards for Antimicrobial Susceptibility Testing MIC Breakpoint (ug/mL) Antimicrobial Agent Test Range (ug/mL) Susceptible Intermediate Resistant Amoxicillin 0.25-128 34 8. >8 Ticarcillin 0.25-128 <8 16 >16 Piperacillin 0.25-128 <8 16 >16 Cefotaxime 0.25-128 <1 2 >2 Ertapenem 0.25-128 30.5 >1 Nalidixic Acid 0.25-128 <8 16 >16 Ciprofloxacin 0.25-128 <0.5 1 >1 Gentamicin 0.25-128 <2 4 >4 Note: For actual MIC Assay, refer to the current Clinical and Laboratory Standards Institute (CLSI) Performance Standards for Antimicrobial Susceptibility Testing available to paid subscribers.
Transcribed Image Text:b. Compare the MIC above with the MIC Breakpoints on the Sample Standards below and fill out the Susceptibility Result column on the table above. Sample Performance Standards for Antimicrobial Susceptibility Testing MIC Breakpoint (ug/mL) Antimicrobial Agent Test Range (ug/mL) Susceptible Intermediate Resistant Amoxicillin 0.25-128 34 8. >8 Ticarcillin 0.25-128 <8 16 >16 Piperacillin 0.25-128 <8 16 >16 Cefotaxime 0.25-128 <1 2 >2 Ertapenem 0.25-128 30.5 >1 Nalidixic Acid 0.25-128 <8 16 >16 Ciprofloxacin 0.25-128 <0.5 1 >1 Gentamicin 0.25-128 <2 4 >4 Note: For actual MIC Assay, refer to the current Clinical and Laboratory Standards Institute (CLSI) Performance Standards for Antimicrobial Susceptibility Testing available to paid subscribers.
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