. Explain the basis for the following statement: Transcription of twogenes on a plasmid can occur without the concomitant action of atopoisomerase, but only if those two genes are oriented in oppositedirections.

Transcription is defined as the process of polymerization of deoxyribonucleic acid (DNA) segment, in…

Answered: . Explain the basis for the following…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

How many statements are right? 1. operator is a protein (transcription factor) that interacts with a DNA sequence immediately downstream the promoter region 2. Type I restriction endonucleases cleave DNA at specific sites, close to recognition sequence 3. Type II restriction endonucleases cleave DNA within or at short specific distances from the recognition site 4. Type IIl restriction endonucleases Cleave DNA at random sites, cleave DNA near the recognition sequence 5. Type IV restriction endonucleases: Target only methylated DNA 3. 1 2. 4. 5.
If an enhancer is placed on one plasmid and its corresponding promoter is placed on a second plasmid that is catenated (linked) with the first, initiation is almost as effi cient as when the enhancer and promoter are on the same plasmid. However, initiation does not occur when the two plasmids are unlinked. Explain.
A sample of E.coli cells is transformed with a plasmid containing a transcriptional fusion including the promoter of an E.coli housekeeping gene and the LacZ gene. The transformed cells are plated out on selective medium containing X-gal. Which of the following best describes the likely results. a. Colonies will only be blue if lactose is also included in the growth medium. b. Colonies will only be blue if lactose and glucose are included in the growth medium c. Most of the colonies should turn blue. d. Presence of any white colonies may indicate deletion or mutation of the promoter. e. Both c. and d. are correct
Assume you have successfully cloned a small (200 bp) fragment of DNA into the polylinker region of a pUC18 cloning vector. Describe the appearance of transformed colonies you would expect to see on each of the following plates: plain media, media containing ampicillin, media containing tetracycline, media containing ampicillin and X-Gal.
Using a flow diagram, elaborate on how you would generate a recombinant plasmid that can be used for the expression of a therapeutic insulin.
A scientist seeks to synthesize a blood clotting factor protein in lab. To perform this experiment,she utilizes recombinant DNA technology where she inserts the gene of interest encoding for theparticular protein from a eukaryotic cell into bacterial plasmids. These plasmids are thesetransformed into E.coli for expression. During structural elucidation of the product, she isdisappointed to discover that the gene product from the bacteria is not the correct protein. Whatcorrective step could she take in the procedure to fix this issue? Justify your answer.
Discuss the role of the regulator in relation with DNA damage and recombination in the article histone h4 dosage modulates DNA damage response in the pathogenic yeast Candida glabrata via homologous recombination pathway questions
. Propose a mechanism by which a type II topoisomerase could use the energy of ATP hydrolysis to scan a large DNA molecule and, thereby, to direct that the enzyme will catalyze largely “disentan- gling" reactions (decatenation and unknotting).
Genetically modified foods are products produced from organisms that have had changes introduced into their DNA using the methods of genetic engineering. Golden rice, Flavr Savr tomatoes and Roundup Ready soyabeans are products of this technology. Discuss the advantages of these three genetically modified crops. Name ONE (1) strategy to produce transformed plant cells.

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. Explain the basis for the following statement: Transcription of two genes on a plasmid can occur without the concomitant action of a topoisomerase, but only if those two genes are oriented in opposite directions.