Lab #10 Report (Redo)
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McKenna Nichols
BME 447
Loeffler
15 November 2023
Chapter 12 Lab Report: Glucose Sensors
Results
Task 1: Glucose Assay Kit with Spectrophotometer
Glucose Concentration
Intensity
Absorbance
200
3582
0.720286
100
11251
0.223221
50
15387
0.087258
0
18811
0
Task 3: Commercial Electrochemical Glucose Sensor
Known Glucose
Concentration
Glucose Reading #1
Glucose Reading #2
Average
300
494
392
443
150
151
149
150
100
111
125
118
50
102
103
102.5
0
0
0
0
Discussion
For the first task of this lab, we began by preparing 5 different solutions each with a
different concentration of glucose. We put 12
each of the solutions into centrifuge tubes as
µ𝐿
well as 1200
of reagent. We then vortexed each solution to mix and then put each solution in
µ𝐿
the heating block for 8 minutes. After allowing the solutions to cool down for 4 minutes, we
placed the solutions into microcuvettes and measured the intensity of each solution at 630 nm.
We then calculated the absorbances of each solution and plotted these values versus the glucose
concentration. This graph can be seen in the results section of this lab report. Our results
indicated that a solution with a higher glucose concentration will have a lower intensity at 630
nm. Based on the relationship between intensity and absorbance, this means that a higher glucose
concentration is indicative of a higher absorbance. The graph may be confusing since the x-axis
is backwards. The relationship shows that an increase in absorbance increases with increasing
concentration.
In the second part of this lab, we began by using a commercial electrochemical glucose
sensor. After inserting a test strip into the meter, we dipped the edge into the solution and
recorded the value produced. We repeated this process twice for all of the solutions we used in
the first part of this lab report. From here we took the average measurement for each solution and
plotted it versus the known glucose concentration. This graph can be seen in the results section
of this lab report. Based on our results, we can conclude that typically the glucose readings from
the commercial electrochemical glucose sensor typically output higher readings than the true
values. The reasoning for this can be seen in the review question below.
Review Questions
12.2
Is there discrepancy between the real concentrations and your meter readings? If so, can you
explain why? Hint: many commercial glucose meters show “plasma equivalent” concentrations
rather than the actual glucose concentrations.
There is a discrepancy between the real concentrations and the meter readings. This is because
the commercial glucose meters use the whole blood sample and provide a reading that reflects
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the “plasma equivalent” whereas the lab readings, which reflect the real concentrations, use
samples that have been processed to remove the red blood cells so therefore the plasma is what is
being tested. These results are more accurate as they are not skewed by the red blood
cells/plasma equivalent blood sample.
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