Lab #10 Report (Redo)

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University Of Arizona *

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447

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Mechanical Engineering

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Jan 9, 2024

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4

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McKenna Nichols BME 447 Loeffler 15 November 2023 Chapter 12 Lab Report: Glucose Sensors Results Task 1: Glucose Assay Kit with Spectrophotometer Glucose Concentration Intensity Absorbance 200 3582 0.720286 100 11251 0.223221 50 15387 0.087258 0 18811 0
Task 3: Commercial Electrochemical Glucose Sensor Known Glucose Concentration Glucose Reading #1 Glucose Reading #2 Average 300 494 392 443 150 151 149 150 100 111 125 118 50 102 103 102.5 0 0 0 0 Discussion For the first task of this lab, we began by preparing 5 different solutions each with a different concentration of glucose. We put 12 each of the solutions into centrifuge tubes as µ𝐿 well as 1200 of reagent. We then vortexed each solution to mix and then put each solution in µ𝐿 the heating block for 8 minutes. After allowing the solutions to cool down for 4 minutes, we
placed the solutions into microcuvettes and measured the intensity of each solution at 630 nm. We then calculated the absorbances of each solution and plotted these values versus the glucose concentration. This graph can be seen in the results section of this lab report. Our results indicated that a solution with a higher glucose concentration will have a lower intensity at 630 nm. Based on the relationship between intensity and absorbance, this means that a higher glucose concentration is indicative of a higher absorbance. The graph may be confusing since the x-axis is backwards. The relationship shows that an increase in absorbance increases with increasing concentration. In the second part of this lab, we began by using a commercial electrochemical glucose sensor. After inserting a test strip into the meter, we dipped the edge into the solution and recorded the value produced. We repeated this process twice for all of the solutions we used in the first part of this lab report. From here we took the average measurement for each solution and plotted it versus the known glucose concentration. This graph can be seen in the results section of this lab report. Based on our results, we can conclude that typically the glucose readings from the commercial electrochemical glucose sensor typically output higher readings than the true values. The reasoning for this can be seen in the review question below. Review Questions 12.2 Is there discrepancy between the real concentrations and your meter readings? If so, can you explain why? Hint: many commercial glucose meters show “plasma equivalent” concentrations rather than the actual glucose concentrations. There is a discrepancy between the real concentrations and the meter readings. This is because the commercial glucose meters use the whole blood sample and provide a reading that reflects
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the “plasma equivalent” whereas the lab readings, which reflect the real concentrations, use samples that have been processed to remove the red blood cells so therefore the plasma is what is being tested. These results are more accurate as they are not skewed by the red blood cells/plasma equivalent blood sample.