BIOS256 Week 4 Lab

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Chamberlain College of Nursing *

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256

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Mechanical Engineering

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Apr 3, 2024

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BIOS256 OL, Week 4 Lab Name: OL Lab 4: Renal Physiology: Find the mode of action of a diuretic drug Learning Objectives:  Understand the morphological relationships between the kidney tubules and the circulatory system  Analyze experimental data for calculating glomerular filtration rate  Set up and perform a perfusion of renal tubules, including the calculation of liquid absorption rates  Learn about the epithelial transport mechanism in the kidney tubules  Use experimental data to assess the mode of action of a diuretic drug Kidneys play a critical role in the filtration and reabsorption of essential substances that are required for the body. In this simulation, you will explore the anatomy of the kidney through 3D holograms and identify the physiological roles in maintaining homeostasis as well as the factors that influence urine regulation. In this simulation, you will use the kidney from a dissected rat to study the mode of action of a new diuretic drug and learn the reabsorption process in the renal tubules. You will learn how to calculate the glomerular filtration rate or GFR and learn the hormones that influence urine output. At the end of the simulation, you should be able to correlate the relationship between renal and circulatory systems in controlling blood pressure. Part 1: Complete Labster- Renal Physiology: Find the mode of action of a diuretic drug Part 2: Report and Reflection Purpose: Describe in your own words and in complete sentences, the purpose of this experiment. The purpose of this experiment is to understand relationships between the kidney tubules and analyze glomerular filtration rate. Observations: List 2 observations you have made in this simulation. The nephron is located in both the cortex and medulla and the glomerulus capillaries push out the largest amounts of solutes.

BIOS256 OL, Week 4 Lab Name: Answer all the questions below: 1. In the simulation, why was the ascending limb of the nephron selected to study the effect of the diuretic drug? (2 points) The ascending limb of the nephron was selected because the first filtration step in the kidneys takes place in the glomerulus. 2. What is renal perfusion and how does it affect blood pressure? (2 points) Renal perfusion helps maintain the amount of urine output. In the case of hypotension renal output is decreased and vice versa. 3. Compare and contrast the cortical and Juxtamedullary nephrons. (2 points) Juxtamedullary nephrons have their loops of Henle dive deeper into the medulla. While cortical nephrons have shorter loops of Henle. 4. What are the hormones that regulate urine output? (2 points) (ADH) Antidiuretic Hormone and Aldosterone hormone 5. What is countercurrent multiplication in a nephron and how does it affect the urine concentration? (2 points) The process of using energy to make an osmotic gradient. Which allows you to absorb water more and produce more urine. 6. Reflection: Reflect on at least 2 key concepts you have learned from this simulation. How can you apply it to human health and diseases? Kidneys filtrate and excrete unnecessary solutes and toxins and are in direct response to urine concentration. The nephrons are utilized in the urine concentration, and it goes from the cortex of the kidneys to the medulla.

BIOS256 OL, Week 4 Lab Name: Grading Rubric: Activity Deliverable Points Part 1 Complete simulation 15 Part 2 Complete lab report and answer questions  Purpose (1 point)  Observation (2 points)  Questions (10 points)  Reflection (2 points) 15 Total Complete all lab activities 30

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