Team Lab Notebook: Plant Metabolic Diversity Project Guide

Exploring Plant Metabolic Diversity Multi-week Team Lab Notebook (TLN) - Lab 4: Weeks 2-4 Team Name: Team #5 Names of team members in attendance: Anna, Lexi, Falco Date: 9/28/23 You will be working on your independent research project over the next three weeks. Each week you should: ● Complete the weekly section of this TLN, save it to the team lab computer and provide a copy of the updated TLN to each team member. ● EACH WEEK one member of the team (preferably the same team member each week) should then upload the updated TLN to the TLN Lab #4 (Weeks 2-4) – Independent Projects assignment dropbox on OAKS. This multiweek TLN will not be graded, but instead is intended to provide you lab instructor with information on the progress of your project. Additionally, just as for other labs, you must get your lab instructor to sign off on the TLN each week, indicating that you have completed it, and cleaned up. ____________________________________________________________________________ ______________ Week 2 – Proposal Development and Peer Review At the end of today’s lab be sure to turn in the following to the indicated assignment dropboxes on OAKS for feedback and grading: ● Submit the following to the Independent Project Proposal Peer Evaluation (Lab 4: Week 2) assignment dropbox. ● The proposal review form that your team completed for the proposal you reviewed. The proposal review form that you completed will be graded based on how thoroughly and accurately you reviewed another team’s proposal. Please also provide a copy (email) of your proposal review to the authoring team. ● Submit the following to the Team Project Proposal (Lab 4: Week 2) assignment dropbox on OAKS: ● Your teams final project proposal, incorporating any changes suggested by the team that evaluated your proposal. ● Submit a copy of the Proposal Peer Review form provided to you by the team that evaluated your proposal. ● If you disagree with specific comments provided by the reviewing teams, provide a justification below for why you chose not to follow their suggestions. Checklist of things to do BEFORE you leave the lab:

● Gas sensor cords are untangled and NOT wrapped around the sensor. Store the sensor at your lab table in its slot in the large biochamber. ● Our lab table and stools are wiped down. ● Gas sensor biochambers and glassware is washed with soap, rinsed, dried and placed back at your lab table. ● All other sensors used are stored in their appropriate cabinet/drawer on the side lab bench. ● If used, the hand-held data loggers (LabQuest2) are turned off & plugged in to the charging station on the side lab bench. ● Trash, used plant material is discarded. ● Class common work area is clean and materials/instruments are returned to their proper place. ● A back-up copy of the team’s data spreadsheet, and this TLN is provided to all team members. ● Complete the Peer Evaluation Form. Turn in hardcopies to your lab instructor or submit the online form to the Peer Evaluation assignment dropbox on OAKS before you leave. ● Submit this TLN to the TLN Lab #4 (Weeks 2-4) – Independent Projects. ● Log off your team computer; the computer should remain on. Your lab instructor must indicate here that your work area is clean before you may leave the lab. Failure to get your instructor to sign off here, can result in a reduction in your project grade! Week 3 – Data Collection Team Name: Team #5 By: (Enter the full names of the team members who are present at today’s lab) Anna, Lexi, Falco Date: 10/5/23 Proper handling, use, and storage of your team’s equipment is critical for its continued proper function. Remember that intentional or careless handling of equipment can result in immediate dismissal from lab, and you may be asked to pay the costs of repairing or replacing equipment. The purpose of today’s TLN is for you to provide your lab instructor with information about your progress on the research project. Note – All teams should be recording their data in a MS Excel Spreadsheet. If you need assistance with organizing your spreadsheet, consult with your instructor. Activity 1: Experimental Protocol and Results Please complete the following: .

1. Describe any modifications you made to your team’s proposal based on feedback from you instructor. If significant changes were made, include the complete revised procedures. We did not need to make any modifications according to the feedback given. 2. At the end of the lab, describe, in detail, how far along your team is in collecting data for your project, and what you have left to complete in your experiment. We have collected data for 8 different House Holly Fern leaves and from 8 different Swamp Laurel Oak leaves. We need to determine which plant has higher CO 2 emissions. 3. Copy and paste a copy of your raw data table from the MS Excel sheet you are using to record your data. Be sure that measurement labels and units of measure are included in the table from your MS Excel Spreadsheet. Dark Reaction House Holly Fern Swamp Laurel Oak Slopes (CO2 PPM per second) -0.2251 0.1371 0.0102 0.19 -0.04837 0.1862 0.01249 0.2637 0.0186 0.2425 -0.005109 0.3669 0.2332 0.1628 -0.02012 0.04354 4. If you were experiencing difficulties with your equipment, please describe the problem. ( Note: malfunctioning equipment should be given to your instructor with a label attached clearly describing the problem.) One of the CO 2 sensors did not read the emissions correctly. Checklist of things to do BEFORE you leave the lab: ● Gas sensor cords are untangled and NOT wrapped around the sensor. Store the sensor at your lab table in its slot in the large biochamber. ● Our lab table and stools are wiped down.

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● Gas sensor biochambers and glassware is washed with soap, rinsed, dried and placed back at your lab table. ● All other sensors used are stored in their appropriate cabinet/drawer on the side lab bench. ● If used, the hand-held data loggers (LabQuest2) are turned off & plugged in to the charging station on the side lab bench. ● Trash, used plant material is discarded. ● Class common work area is clean and materials/instruments are returned to their proper place. ● A back-up copy of the team’s data spreadsheet, and this TLN is provided to all team members. ● Each person completed the Peer Evaluation Form and submitted it to the Peer Evaluation Form assignment dropbox on OAKS. ● Submit this updated TLN to the TLN Lab #4 (Weeks 2-4) – Independent Projects. ● Log off your team computer; the computer should remain on. Your lab instructor must indicate here that your work area is clean before you may leave the lab. Failure to get your instructor to sign off here, can result in a reduction in your project grade! Week 4 – Data Collection and Analysis Team Name: 5 By: (Enter the full names of the team members who are present at today’s lab) Anna, Lexi, Falco Date: 10/12/23 Proper handling, use, and storage of your team’s equipment is critical for its continued proper function. Remember that intentional or careless handling of equipment can result in immediate dismissal from lab, and you may be asked to pay the costs of repairing or replacing equipment. The purpose of today’s TLN is for you to provide your lab instructor with information about your progress on the research project. Experimental Results Please complete the following: . 1. At the end of lab, describe, in detail, how far along your team is in collecting data for your project. If your team still needs to collect additional data, you will need to schedule a time to come to lab to finish. Consult with your instructor if this is the case.

We finished data collection last week. We did the data collection in a series of 8 different trials for each plant, Holly Fern & Laurel Oak. In total it was 16 different trials. 2. If you were experiencing difficulties with your equipment, please describe the problem. ( Note: malfunctioning equipment should be given to your instructor with a label attached clearly describing the problem.) No problems. 3. Copy and paste a copy of your raw data table below. Be sure that measurement labels and units of measure are included in the table from your MS Excel Spreadsheet. Dark Reaction House Holly Fern Swamp Laurel Oak Slopes (CO2 PPM per second) -0.2251 0.1371 0.0102 0.19 -0.04837 0.1862 0.01249 0.2637 0.0186 0.2425 -0.005109 0.3669 0.2332 0.1628 -0.02012 0.04354 Analysis 4. Summarize the results of your statistical analyses below. ONLY indicate the statistical tests you performed, your null and alternative hypotheses, test statistics (p value(s), test statistic, sample size) and your conclusions regarding the null hypothesis. Do not copy and paste the statistical test results table from Excel! We performed a T-test of equal variance. The null hypothesis was that there was no difference between CO 2 emissions in the two different species of plants. The alternative hypothesis, which is what we accepted, was that there is a difference between the CO 2 emissions in the two different species of plants. The sample size was 16, which is 8 of each plant type. The p-value was

0.0209093842807205, which is less than 0.05. This concludes again, why we rejected the null hypothesis. 5. Work together as a team to develop appropriate graphs summarizing your data. Copy and paste (as a jpeg image) the graph(s) your team develops. Below your graph, agree as a team on some of the points you would like to make about your data and analysis. There are numerous video tutorials on OAKS that will help you with constructing a graph in MS Excel. Consult Appendix E in the lab manual for tips on writing the text of the results. The data presented in the graph above indicates the means of the data from our test trials. A two sample- assuming equal variances t-test revealed that the Swamp Laurel Oak CO2 emissions were significantly greater than the House Holly Fern CO2 emissions. Discussion/Conclusions 6. Develop an outline of the topics that will be discussed in the Discussion section of the draft independent projects article. Consult Appendix E in the lab manual for tips on writing the discussion. (Note – you may each use this outline to write your discussion but remember that the discussion section of each person’s article should be written in your own words, according to the guidelines on writing in this class explained in the Common Lab Syllabus posted on OAKS.

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Outline for discussion: - Start with a brief overview of our experiment - Discuss the results of the experiment - Discuss how our data supports our hypothesis - Discuss how our data is consistent with the original prediction that the House Holly Fern emitted more CO2 - Discuss specific results that led us to make our argument - Connect conclusions to what you've learned about the biology of an organism (make an argument as to why this occurred- do not just state your hypothesis) - Use background research to support the argument - Discuss possible alternative conclusions (other factors that may have caused the results) - Discuss how the data supports these alternative conclusions - Describe limitations of our experiment - Discuss any problems we ran into that may have influenced our results - Speculate on different methods that could be used that would fix any issues we ran into 7. Our team decided that it will be necessary for each team member to research the following topics in order to write the individually written portions of the draft article (abstract, introduction & discussion/conclusions). Feel free to begin this research in lab using your textbook, trustworthy internet sources or articles from related biological science professional journals and share your findings with your teammates. - Anna will be researching why some plants give off more CO2 emissions in low light than other species of plants - Lexi will research how our experiment is relevant to biology-related issues - Falco will be researching the difference in CO2 emissions of both Swamp Laurel Oak and holly fern in low light situations. Checklist of things to do BEFORE you leave the lab: ● Gas sensor cords are untangled and NOT wrapped around the sensor. Store the sensor at your lab table in its slot in the large biochamber. ● Our lab table and stools are wiped down.

● Gas sensor biochambers and glassware is washed with soap, rinsed, dried and placed back at your lab table. ● All other sensors used are stored in their appropriate cabinet/drawer on the side lab bench. ● If used, the hand-held data loggers (LabQuest2) are turned off & plugged in to the charging station on the side lab bench. ● Trash, used plant material is discarded. ● Class common work area is clean and materials/instruments are returned to their proper place. ● A back-up copy of the team’s data spreadsheet, and this TLN is provided to all team members. ● Each person completed the Peer Evaluation Form and submitted it to the Peer Evaluation Form assignment dropbox on OAKS. ● Submit this updated TLN to the TLN Lab #4 (Weeks 2-4) – Independent Projects. ● Log off your team computer; the computer should remain on. Your lab instructor must indicate here that your work area is clean before you may leave the lab. Failure to get your instructor to sign off here, can result in a reduction in your project grade!

Exploring Plant Metabolic Diversity

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