PhysioEx Exercise 1 Activity 4

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Chemistry

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Jan 9, 2024

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PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 4: Simulating Filtration Name: Jackie Chau Date: 14 September 2023 Session ID: session-90d13d2b-01b7-eaf1-f72e-38373976f877 Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Experiment Results Predict Questions Filtration is a process that You correctly answered: is passive. 1 Filtration is dependent upon a You correctly answered: hydrostatic pressure gradient. 2 The fi ltrate You correctly answered: All of these answers are correct. 3 An important place that fi ltration takes place in the body is in You correctly answered: the kidneys. 4 Predict Question 1: What e ff ect will increasing the pore size of the fi lter have on the fi ltration rate? Your answer: The fi ltration rate will increase. 1 Predict Question 2: What will happen if you increase the pressure above the beaker (the driving pressure)? Your answer: The fi ltration rate will increase. 2
Stop & Think Questions Experiment Data Run Number Solute MWCO Pressure (mm Hg) Filter Rate (ml/sec) Residue Start Conc. (mg/ml) Filter Conc. (mg/ml) 1 Na Cl 20 50 1.00 present 5 0.00 1 Urea 20 50 1.00 present 5 0.00 1 Glucose 20 50 1.00 present 5 0.00 1 Powdered Charcoal 20 50 1.00 present 5 0.00 2 Na Cl 50 50 2.50 present 5 4.81 2 Urea 50 50 2.50 present 5 0.00 2 Glucose 50 50 2.50 present 5 0.00 2 Powdered Charcoal 50 50 2.50 present 5 0.00 3 Na Cl 200 50 10.00 present 5 4.81 3 Urea 200 50 10.00 present 5 4.74 3 Glucose 200 50 10.00 present 5 4.39 3 Powdered Charcoal 200 50 10.00 present 5 0.00 4 Na Cl 200 100 20.00 present 5 4.81 4 Urea 200 100 20.00 present 5 4.74 4 Glucose 200 100 20.00 present 5 4.39 4 Powdered Charcoal 200 100 20.00 present 5 0.00 Post-lab Quiz Results The reason none of the solutes were present in the fi ltrate was that You correctly answered: the solutes were all too large to pass through. 1 What does an increase in the driving pressure correspond to in the body? You correctly answered: an increase in blood pressure. 2 + - + - + - + -
You scored 100% by answering 4 out of 4 questions correctly. Review Sheet Results After fi ltration, substances that pass through the fi lter are called the fi ltrate, which includes You correctly answered: all of the above. 1 The top beaker in the simulation corresponds to You correctly answered: the blood capillary. 2 Why was there not 100% recovery of the Na Cl solute with any of the membranes? You correctly answered: Some of the solute remained on the membrane fi lter. 3 + - An increase in blood pressure would probably initially __________ fi ltration in the kidneys. You correctly answered: increase the rate of. 4 Explain in your own words why increasing the pore size increased the fi ltration rate. Use an analogy to support your statement. How well did the results compare with your prediction? Your answer: Increasing the pore size of the fi lter increases the fi ltration rate because it will allow more solutes and solvent to pass through to the other side of the membrane. It is very similar to if you were trying to let 10 people through a big car garage door where all of them can enter all at once to get into the house while the front door would only let only one person in at a time. 1 Which solute did not appear in the fi ltrate using any of the membranes? Explain why. Your answer: One solute that did not appear in the fi ltrate was the powdered charcoal. I think it did not appear in any of the membranes because it is much larger than any of the 2
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membrane pores used in the experiment. Therefore it would not have been able to pass through to be a part of the fi ltrate past the fi lter. Why did increasing the pressure increase the fi ltration rate but not the concentration of solutes? How well did the results compare with your prediction? Your answer: The reason why increasing the pressure will increase the rate of fi ltration but not the concentration of solutes is because you are pretty much just pushing the solvent through the fi lter to fi lter at a faster rate but you can not alter the size of the solutes being fi ltered out unless you want to modify the structures of it. For example, the powdered charcoal was always fi ltered out of the fi ltrate for all parts of the experiment no matter how much pressure was applied, because it was just too big than the available pores in the experiment, it was always fi ltered out no matter what. 3