Lab 6

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Florida International University *

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2000L

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Chemistry

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Jan 9, 2024

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Laboratory Report for Protein Synthesis and Digestion Student Name: Sachintha Peiris Date: 10/18 TA Name: Lukkamol Prapkree D. Data Record (20 points) Please attach below your original DNA sequence provided by your TA by stapling it (folded) in the box below (If you do not provide your original DNA sequence, you will lose 20 points). GTC CCG TAC TTA CGA AAC GAG CGA TCG CAT ACT 1. Please provide the corresponding mRNA sequence for the DNA sequence of your protein molecule (5 points). CAG GGC AUG AAU GCU UUG CUC GCU AGC GUA UGA 2. Where does DNA transcription simulated above take place in the cell? Nucleus (1 pt) 3. How many codons ( after start codon ) are there in your RNA sequence? 10 (2 pts) 4. Use the amino acid/codon information provided in Table 1 to determine the amino acid sequence of your polypeptide/protein. Please detail in the space provided below the amino acids present in your protein. Example: Phe – Try- Gln – Pro – etc., etc. (5 points) Gin- Gly-Met-Asn-Ala-Leu-Leu-Ala-Ser-Val-Stop 5. How many peptide bonds does your polypeptide chain contain? 8 (1 pt) 6. Where does RNA translation into polypeptides take place in the cell? Ribosome (1 pt) 7. Name at least three components of cell machinery, molecules, or organelles that are necessary for RNA translation/Protein synthesis to take place (2 pts): ribosomes, mRNA, and tRNA 8. How would stop codons affect the translation process for this polypeptide? Did your sequence contain any stop codons? If so, what were they? (3 pts) Stop codons signal the termination of the process by binding release factors, my sequence had one stop codon at the end: UGA.
E. Digestion Data Record and Analysis (10 points) 1. In the space provided below, please write the treatment for each control and experimental well on your gelatin plate. Predict how much digestion you expect in each well based on the treatment for that well on a scale of (-) no digestion to (+++) significant digestion (5 pts). 1. Water: - 2. Water: - 3. Protease: + 4. Protease: + 5. Trypsin: ++ 6. Trypsin: ++ 7. HCL: +++ 8. HCL: +++ 9. Unknown 1 : ++ 10. Unknown 1I: ++ 11. Unknown 2: +++ 12. Unknown 2: +++ 2. Please complete the table below for your Protein Digestion experiment. List the well number, treatment used, initial diameter (should be the same for all wells), and final diameter (mm). Score the digestion in each well using the following scale: +++ significant digestion (increase of 2mm or more), ++ moderate digestion (increase of at least 1mm), + some digestion (increase of less than 1 mm in diameter), and – no digestion occurred. (5 pts) Well Number Treatment Initial Diameter (mm) Final Diameter (mm) Change in Diameter (mm) Digestion Score 1 Water 10 10 - no 2 Water 9 9 - no 3 Protease 10.5 10 + some 4 Protease 10 10 - no 5 Trypsin 8 10 +++ significant 6 Trypsin 10 10 - no 7 HCL 10 12 +++ significant 8 HCL 10 11 ++ moderate 9 Unknown 1 11 10 ++ moderate 10 Unknown 1 10 9 ++ moderate 11 Unknown 2 10 8 +++ significant 12 Unknown 2 10 8 +++ Significant
F. Discussion Questions (20 points) 1. Describe in detail the sequence of events that takes place within a cell when a polypeptide chain is synthesized (DNA RNA Protein). Be specific . (5 pts) In the nucleus of the cell DNA is transcribed into mRNA. Messenger RNA (mRNA) sequences are then transcribed in the nucleus then move into the cytoplasm. Here they are translated into s specific amino acid sequence corresponding with a particular polypeptide chain. The mRNA nucleotide sequence is then matched up with a specific amino acid, forming a peptide bond. This continues until the polypeptide chain is completely formed. l 2. List at least three different proteases you learned about in this lab exercise. Where does each one digest protein within the gastrointestinal tract? (5 pts) Lipase is in the pancreas and helps digest lipids and fatty acids. Pepsin digests protein with acidity in the stomach. Trypsin is in the small intestine due to the actions of the pancreas. 3. Explain what happened in the gelatin wells exposed to your two unknown solutions. Did digestion take place? If so, explain why it would be beneficial to have protein-digesting additives (namely enzymes) in household cleaners and detergents. (5 pts) Digestion did take place, because the diameter decreased. It would be beneficial to have protein-digesting additives in house hold cleaners because messes often contain food that can be digested by these additives, aiding with cleaning. 4. Summarize what the 24-hour recall earlier in the semester revealed about your personal protein intake. What high-protein foods did you consume during that 24-hour period? Did your protein intake match the DRIs? Be specific . (5 pts) The 24-hour recall earlier in the semester revealed that my personal protein intake was adequate. During the 24 hours period consumed meat, eggs, nuts, and protein bars, these helped me reach my DRI. My intake matches my DRI.
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