Starch Assignment (2)
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Wayne State University *
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Course
2220
Subject
Chemistry
Date
Apr 3, 2024
Type
docx
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2
Uploaded by JusticeLightningGoat38
Name: Valeria Diaz
Lab Report: Starch
I
nstructions
Step 1:
After reading the lab manual and powerpoint, and watching the video provided on Canvas, complete the following table. (5 points) Step 2:
Answer the study questions. Please answer questions thoroughly. (5 points) After completing the lab based on the lab manual instructions and watching the lab video provided on canvas, answer the following questions. After performing Agitation and temperature effect on the smoothness of the paste (Part 1) of the procedures:
1.
describe the consistency of the sol from the procedure A-C based on the experiment. 2.
Describe only the appearance
of each corn starch pastes after performing part 2 of the procedures and after watching the video titled “Starch Video” under modules. This is a YouTube video. Starch
Appearance
Potato Starch a thickened gel
Waxy Rice Starch liquid, cloudy
Rice Starch liquid cloudy
Corn Starch 16g
thickened liquid
Corn Starch 8g
semi-thick liquid
Tapioca Starch
clear, semi-jelly
3.
Use part 3 of the procedures to perform a cold line spread and provide the average of each sample given in the video. Starch
Cold Line Spread
L
M
N
O
P
Study Questions:
1.
Why do some experiments form lumps in the starch paste during the process (refer to results from part 1 of the procedures in the lab manual)?
Starch clumps tend to clump together due it being hydrophobic
2.
Describe how the amylose content affects the properties of starch paste (refer to the lab manual).
It can affect the texture of the paste
3.
Provide examples of starch that are suitable for making thick soups.
Cornstarch is typically the best for making thick soups
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Related Questions
read the Procedure and answer the post lab questions below:
Using a balance, mass between 1.50 – 2.00 grams of sodium carbonate in a pre-massed 150mL beaker.
Add 20 mL of distilled water and stir thoroughly to make sure all the crystals are dissolved. Rinse the stirring rod with a little distilled water after stirring.
Using a balance, mass between 1.50 – 2.00 grams of calcium chloride dihydrate in a pre-massed 50 mL beaker.
Repeat Step 2 for the solution in the 50 mL beaker.
Pour the calcium chloride solution into the 150mL beaker containing the sodium carbonate solution and stir.
Mass a piece of filter paper. Fold the filter paper and place it into the funnel. Wet it with a little distilled water to ensure that it is stuck to the sides of the funnel.
Slowly pour the mixture from the beaker into the funnel and collect the filtrate into a 125mL erlenmeyer flask. Do not allow the mixture to rise above the edge of the filter paper.
Rinse the beaker with about 10 mL of distilled water.…
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Color BEFORE adding
Color AFTER adding
Intensity of color AFTER
adding FeCl3
Test Tube #
FeCl3
FeCl3
#1 (salicylic acid)
clear
lilac
100%
#2 (commercial aspirin A) clear
slightly pink
50%
#3 (commercial aspirin B) clear
lilac
95%
#4 (aspirin from your
clear
pink
20%
synthesis above)
#5 (control)
clear
clear
0%
Based on this data and the melting point of Aspirin, how pure is the synthesized product?
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Use special gel loading tips or a micro-syringe to load the complete sample in a narrow well. Take care not to poke the well bottom with the tip as this will create a distorted band. Never overfill wells. This could lead to poor data if samples spill into adjacent wells, and poorly resolved bands. Load 20-40 μg total protein per mini-gel well.
-If you have a lysate with protein concentration of 1500 ug/mL, how much sample you need to load according to the step indicated in D.3
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. Be sure to understand what the dilutionfactor is for each sample (dilution factor = the number used to determine the actualconcentration of the stock UK after the diluted samples are analyzed) ?
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Using the data table below- calculate the concentration of the albumin in each of the test tubes labeled 1 to 6,determine the total volume in each of the test tubes. Prepare a well labeled best fit line graph using the absorbance given, and using the given absorbance of the unknown sample 7 determine the unknown protein concentration. Submit the graph of the data to your instructor electronically following the instructions provided by your instructor.
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1 and 2 answers?
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A sample of rainwater was collected from four separate locations across theMetroplex. Each sample was measured once by two separate methods for the presence ofsulfate. Determine whether the two methods are equivalent with respect to giving thesame answer for [SO42-].
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Soil Lab 11a Direct Measurement of Soil Porosity
1. Fill a 100 ml graduated cylinder to the 100 ml mark with tap water. Tilt the beaker containing the
soil sąved from Step 3 in the Bulk Density Determination to about a 45 degree angle. Pour the water
very slowly onto the soil in the beaker. Stop adding water when the soil pores are filled (saturated)
and the soil surface glistens.
2. Allow the soil to stand for several minutes to make sure the water has filled the very small soil pores.
Any excess water can be returned to the graduated cylinder. Don't stir or shake the mixture.
3. Determine the amouht of water used to exactly fill the pote space in the 100 cubic cehtimeters of
soil. Subtrąct the reading of water level on the side of the graduated cylinder from the original
volume of 100 ml. Note that the water which was poured onto the soil exactly filled the pore space
and thus equals the total pore volume by direct measurement.
4. Record your results below and make the necessary…
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Data Table 1
Enter the calculated molarity and measured conductivity values in the table.
Data Table 1: Experimental Data
Sodium
Total
Sodium Chloride
Standard Chloride Volume Concentration (M or Conductivity (µS)
(g)
(mL)
mol/L)
Blank
100.00
8.965
A
0.0059 100.00
В
0.0531 100.00
0.1029 100.00
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For this statement, answer True or False and explain your answer briefly.
Polarity is the only characteristic used in liquid-liquid extraction to isolate a poison from the biological matrix.
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PART 3- LABORATORY EQUIPMENT
EQUIPMENT NAME
FUNCTION
To hold test tubes.
Attaches to a retort stand to support glassware being heated.
To pour liquid from a large container into a small container.
To provide a flame during an experiment.
ones
To light the Bunsen burner.
To attach test tubes to a retort stand.
To clean substances out of a test tube.
To hold a hot crucible or flask.
To hold a hot test tube.
To place on the lab bench to prevent burning or to use with ring clamp.
To transfer small amounts of liquid.
To grow bacteria or contain small experiments.
To hold liquids.
To measure liquids.
To contain experiments (a stopper can be used to close the flask).
To hold a hot beaker.
To perform an experiment with a different substance in each well.
To hold chemicals and perform experiments in.
To scoop and transfer small amounts of substances,
To stir during experiments that involve mixing or dissolving.
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Compound
Phthalic Acid
*Formula:
C ₂ H 6 Ou
Naphthalene
Water
Methanol
Structure
+01
with purity
Pu
ON
Mol
(g/m
Procedure:
1. Prepare Hot Water for Recrystallizations
16
(
12
All data and observation taken during lab should
with a single line so the original is still legible.
Add-100 mL of water to a 125-mL Erlenme
Add a boiling stone to prevent bumping dur
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Table #2 Worktable for BCA Assay
Standard
Water (μl)
Tube #
1
2
3
4
5
6
7
8
9
0
125
325
175
325
325
325
400
400
BSA source (μl)
300 stock
375 stock
325 stock
175 of tube #2
325 of tube #3
325 of tube #5
325 of tube #6
100 of tube #7
0
Protein (ug)
Add the volumes listed in Table 2 to each tube. Add the water first and then the
appropriate standard. Pipette each of the protein samples directly into the water, do not
dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and
contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each
of the tubes and write this in Table 2 (Protein (ug)).
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A gram of soil sample as received contains 15.00% water. After oven drying to remove the mixture, it was found to contain 16.00% K. Find the %K in the original sample as received.
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Answer the question from picture, please
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1. Calculate the exact concentration of the stock protein solution in (mg / mL) based on the exact mass of albumin and the volume of the vial used to prepare the broth.
0.4905 g of albumin in 500 mL volumetric flask (mass of protein used to prepare the stock
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Related Questions
- read the Procedure and answer the post lab questions below: Using a balance, mass between 1.50 – 2.00 grams of sodium carbonate in a pre-massed 150mL beaker. Add 20 mL of distilled water and stir thoroughly to make sure all the crystals are dissolved. Rinse the stirring rod with a little distilled water after stirring. Using a balance, mass between 1.50 – 2.00 grams of calcium chloride dihydrate in a pre-massed 50 mL beaker. Repeat Step 2 for the solution in the 50 mL beaker. Pour the calcium chloride solution into the 150mL beaker containing the sodium carbonate solution and stir. Mass a piece of filter paper. Fold the filter paper and place it into the funnel. Wet it with a little distilled water to ensure that it is stuck to the sides of the funnel. Slowly pour the mixture from the beaker into the funnel and collect the filtrate into a 125mL erlenmeyer flask. Do not allow the mixture to rise above the edge of the filter paper. Rinse the beaker with about 10 mL of distilled water.…arrow_forwardColor BEFORE adding Color AFTER adding Intensity of color AFTER adding FeCl3 Test Tube # FeCl3 FeCl3 #1 (salicylic acid) clear lilac 100% #2 (commercial aspirin A) clear slightly pink 50% #3 (commercial aspirin B) clear lilac 95% #4 (aspirin from your clear pink 20% synthesis above) #5 (control) clear clear 0% Based on this data and the melting point of Aspirin, how pure is the synthesized product?arrow_forwardUse special gel loading tips or a micro-syringe to load the complete sample in a narrow well. Take care not to poke the well bottom with the tip as this will create a distorted band. Never overfill wells. This could lead to poor data if samples spill into adjacent wells, and poorly resolved bands. Load 20-40 μg total protein per mini-gel well. -If you have a lysate with protein concentration of 1500 ug/mL, how much sample you need to load according to the step indicated in D.3arrow_forward
- . Be sure to understand what the dilutionfactor is for each sample (dilution factor = the number used to determine the actualconcentration of the stock UK after the diluted samples are analyzed) ?arrow_forwardUsing the data table below- calculate the concentration of the albumin in each of the test tubes labeled 1 to 6,determine the total volume in each of the test tubes. Prepare a well labeled best fit line graph using the absorbance given, and using the given absorbance of the unknown sample 7 determine the unknown protein concentration. Submit the graph of the data to your instructor electronically following the instructions provided by your instructor.arrow_forward1 and 2 answers?arrow_forward
- A sample of rainwater was collected from four separate locations across theMetroplex. Each sample was measured once by two separate methods for the presence ofsulfate. Determine whether the two methods are equivalent with respect to giving thesame answer for [SO42-].arrow_forwardSoil Lab 11a Direct Measurement of Soil Porosity 1. Fill a 100 ml graduated cylinder to the 100 ml mark with tap water. Tilt the beaker containing the soil sąved from Step 3 in the Bulk Density Determination to about a 45 degree angle. Pour the water very slowly onto the soil in the beaker. Stop adding water when the soil pores are filled (saturated) and the soil surface glistens. 2. Allow the soil to stand for several minutes to make sure the water has filled the very small soil pores. Any excess water can be returned to the graduated cylinder. Don't stir or shake the mixture. 3. Determine the amouht of water used to exactly fill the pote space in the 100 cubic cehtimeters of soil. Subtrąct the reading of water level on the side of the graduated cylinder from the original volume of 100 ml. Note that the water which was poured onto the soil exactly filled the pore space and thus equals the total pore volume by direct measurement. 4. Record your results below and make the necessary…arrow_forwardData Table 1 Enter the calculated molarity and measured conductivity values in the table. Data Table 1: Experimental Data Sodium Total Sodium Chloride Standard Chloride Volume Concentration (M or Conductivity (µS) (g) (mL) mol/L) Blank 100.00 8.965 A 0.0059 100.00 В 0.0531 100.00 0.1029 100.00arrow_forward
- For this statement, answer True or False and explain your answer briefly. Polarity is the only characteristic used in liquid-liquid extraction to isolate a poison from the biological matrix.arrow_forwardPART 3- LABORATORY EQUIPMENT EQUIPMENT NAME FUNCTION To hold test tubes. Attaches to a retort stand to support glassware being heated. To pour liquid from a large container into a small container. To provide a flame during an experiment. ones To light the Bunsen burner. To attach test tubes to a retort stand. To clean substances out of a test tube. To hold a hot crucible or flask. To hold a hot test tube. To place on the lab bench to prevent burning or to use with ring clamp. To transfer small amounts of liquid. To grow bacteria or contain small experiments. To hold liquids. To measure liquids. To contain experiments (a stopper can be used to close the flask). To hold a hot beaker. To perform an experiment with a different substance in each well. To hold chemicals and perform experiments in. To scoop and transfer small amounts of substances, To stir during experiments that involve mixing or dissolving.arrow_forwardCompound Phthalic Acid *Formula: C ₂ H 6 Ou Naphthalene Water Methanol Structure +01 with purity Pu ON Mol (g/m Procedure: 1. Prepare Hot Water for Recrystallizations 16 ( 12 All data and observation taken during lab should with a single line so the original is still legible. Add-100 mL of water to a 125-mL Erlenme Add a boiling stone to prevent bumping durarrow_forward
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SEE MORE QUESTIONS
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ISBN:9781285199047
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