Experiment 2A
pdf
keyboard_arrow_up
School
California State University, Long Beach *
*We aren’t endorsed by this school
Course
223A
Subject
Chemistry
Date
Feb 20, 2024
Type
Pages
4
Uploaded by ChancellorStarSalamander23
13 Experiment 2A: TLC Analysis of Analgesic Drugs Instruction Sheet You will need to bring to your lab section: 1.
A lab notebook with procedures written in your own words 2.
‘Making the Connections’ text
3.
A HARD COPY of this document (3 pages Worksheet) 4.
A RULER (with cm scale) Tips
for effective time management: •
You can use the time spent developing TLC spots to work on questions. Worksheet/Report
: Found in the next three pages. This is due at the end of the lab period. NO other reports need to be submitted besides the Worksheet.
14 Experiment 2A: TLC Analysis of Analgesic Drugs Worksheet Lab Section ____________ Last Name ____________First_________ Date: ____________ Lab Partner’s Name
________________ Unknown Code (A, B, C, …)
________ (20 Points Possible) TLC results: Part 1)
Use the plates below to draw a representation of the TLC plates: Each plate should contain the standard solutions and the right plate for your unknown. Clearly show how each measurement was determined by a ruler for the calculation of R
f
values. (2 points) Part 2)
Report and calculate the R
f
values for each of the spots. (4 points, 1 point for each blank) Eluent
(Solvent Mixture) used to develop plate: ____________________________ Compound Spotted R
f
values Aspirin Ibuprofen Your unknown
15 Part 3)
Which analgesic drug was your unknown? (1 point) Part 4)
Is your unknown pure? What is the evidence of its purity (or contamination)? (1 point) Part 5)
Change the Eluent type to Ethyl Acetate: Hexane (50:50),
use the plate below to draw a representation of the TLC plate. The plate should contain a mixture of the standards
for comparison. (1 point) Part 6)
Report the R
f
values of Part (5): (1 point) Compound Spotted R
f
values Aspirin Ibuprofen Your unknown Part 7)
Compared to Day 1 (Worksheet Part 2), does this change in solvent type in Part (5) affect the R
f
values? Rationalize your observations. (2 points) Part
8)
(a) In addition to the solvent mixture chosen as Eluent in this experiment, name the solvent that was used to create the spotting solution of your unknown. (1 point) (b) Does the solvent in Part 8(a) affect the R
f
values in this entire experiment? Briefly explain. (1 point)
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
- Access to all documents
- Unlimited textbook solutions
- 24/7 expert homework help
16 Additional Questions: 1) A mixture of benzoic acid, para-xylene and benzaldehyde is spotted on a silica gel TLC plate and developed using Ethyl Acetate with 0.5% acetic acid as the solvent. Predict the relative R
f
values for the three components in the sample (highest to lowest). (2 points) 2) Arrange the following solvents in order of increasing polarity (least polar to most polar): (2 points) Diethyl Ether, Propyl Amine, 3-Methyl-Pentane, Acetone 3) If you ran an unknown sample on a TLC using 3-Methyl-Pentane as eluent and observed only one spot with a Rf value ~0.05, is it safe to assume that it is a pure compound? If not, how can you check it out using TLC? (2 points)
Related Documents
Related Questions
I
Part 3 Discussion questions: Please answer in less than 2 paragraphs (20 points)
1. The FDA scientists have published 2 papers on the subject of sunscreen dermal
absorption. What is different between the publications? The editorial page in the journal
JAMA (Journal of American Medication Association) might help you answer this question.
arrow_forward
Think about the situation where you are investigating the relationship of study time vs. exam grade.a. Which variable would be on the x-axis (and why)?b. Which variable would be on the y-axis (and why)?c. Would you expect the slope to be positive or negative (and Why)?
2) Contrast and compare interpolation with extrapolation. Which is “safer” to use and why?3) Look up how “the best fit straight line” is constructed. The term “Least squares” should be in your explanation.4) If I plot some data and end up with a r2 of (-) 0.99, is my plot useless? Explain.
arrow_forward
Pre-Lab Questions
Experiment 12
Food Chromatography: The Distance
Our Food Travels
Name:
Section:
Instructor:
Date:
Imagine you have a solution that is comprised solely of component A. You have another solution that
is comprised solely of component B, and yet a third solution that is comprised solely of component C.
Component A's retention factor is about 0.84, Component B's retention factor is about 0.21, and
Component C's retention factor is 0.55 in the same solvent system. You then have a fourth solution that
is composed of components A and C.
145
You spot each solution on a piece of chromatography paper and allow the solutions to separate. Sketch
what you think your paper would look like below.
arrow_forward
Please help
arrow_forward
How long is a microcentury, if a lecture period (50.0 min) is close to 1.00 microcentury?
arrow_forward
What are the advantages of a microscale lab?
arrow_forward
Question: Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected.
My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC.
-Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.
arrow_forward
What can labs do to reduce error in GC- ms??
arrow_forward
Instructions You are required to produce a short lab report using the information given in the accompanying document, ‘Lab report assessment_student data’. This document introduces an experiment, and also provides a set of experimental data for this experiment. You are required to use this data to perform steps 8 and 9 of the procedure. This includes calculations of a particular value, and then comparison to the true value. Consider any experimental errors when comparing calculated values and true values. Your report should be between 300 and 500 words (including all table contents and titles of tables and graphs). Your lab report should consist of three sections only: • Experimental • Results and Discussion • Conclusion You do NOT need to include an introduction section. Your experimental section should be written in appropriate lab report format, (passive voice, 3 rd person, in paragraphs). Your results and discussion section should contain the data presented in table format, and…
arrow_forward
There is one incorrect answer here. Can you help me figure out which one?
arrow_forward
Using the data provided please help me answer this question.
Determine the concentration of the iron(Ill) salicylate in the unknown directly from to graph and from the best fit trend-line (least squares analysis) of the graph that yielded a straight line.
arrow_forward
why is it importnant to write a lab report once the experiment is finished
arrow_forward
Multiple Choice
Each of the numbered items or incomplete statements is followed by answers or by completions of the statement.
Select the one lettered answer or completion that is best in each case. Write your answer before each number.
27.Quality control department maintain and store records that represent the history of batch from start to finish
a. Quality Coordinating office
b. Analytical Laboratory
d. Material Inspection Section
e. Biological Testing Laboratory
C.
Specification & Analyrtical Development section
28. Which of the following cannot be assay by direct titration with standard base?
Citric acid in calamansi juice
a. Magnesium hydroxide in Milk of Magnesia C.
Acetic acid in vinegar
b.
d.
Diluted phosphoric acid
29. This is used for quantitative preparation of a standard solution:
a.
Pipet
C.
Volumetric flask
b. Graduated cylinder
d.
Buret
arrow_forward
in text form with proper workings and explanation for each and every part and steps with concept and introduction no AI no copy paste remember answer must be in proper format with all working!!!!!!!
arrow_forward
10
arrow_forward
Suspect #1: George Norman
Suspect #2: Becky Baker
George Norman recently
Becky Baker is a world famous
chef. She had just finished
baking a cake at the time of
the crime. The recipe called
for baking soda. The formula
for baking soda
(sodium
bicarbonate) is
had an accident
where he cut
himself with a
Alaia
knife. He cleaned
the wound with
rubbing alcohol.
The formula for
rubbing alcohol
Bak ing
Seda
NaHCOз.
(isopropanol) is C3H80.
Suspect # 3: Liz Lemon
Suspect #4: Ben Linus
Liz Lemon just had her nails
Ben Linus had been walking
done, and she still has
traces of nail polish remover
on her hands. Nail
around in the rain
for about an hour
before the crime
polish remover
he was suspected
contains acetone.
Acetone
of occurred. He
The formula for
Polish
was dripping wet with water
acetone is C3H60.
renover
at the time. The formula for
water is H2o.
arrow_forward
Styles
Voice
Text in Portuguese is not being checked. Do you
(i) MISSING PROOFING TOOLS want to download proofing tools and future
Sensitivity
Editon
Download
updates?
一
4
7.
10 1
11
| 12
17. What is the main side effect of anti-epileptic drugs?
a. Hypertension
b. Drowsiness
c. Tachycardia
I.
d. Insomnia
e. Euphoria
I believe these drugs are like the ones of the work- dihidropyridines-cont
18. What is metformin used for as first choice of treatment, and why?
a. Type II diabetes, because it regulates blood glucose levels
b. Hyperthyroidism, because it inhibits the formation of T3 an
idirm hecause it doesn't interact with other drug
9.1
7.
8.
arrow_forward
Using the lab instructions and data table with data, please help me complete part 1 and 2 of my lab worksheet
arrow_forward
ed Test
This test has a time limit of 1 hour.This test will save and submit automatically when th
Warnings appear when half the time, 5 minutes, 1 minute, and 30 seconds remain.
tiple Attempts Not allowed. This test can only be taken once.
ce Completion This test can be saved and resumed at any point until time has expired. The timer will
emaining Time: 57 minutes, 15 seconds.
uestion Completion Status:
A Moving to another question will save this response.
Question 4
The standard Gibb's Free Energy (AGO) = 18.38 kJ at 25C for the reaction
HNO2 2 H+ + NO2-.
What is the value of AG if {HNO2} = 0.75 M, {H+} = 0.13M and {NO2-} = 0.07M?
%3D
%3D
%3D
Is the reaction spontaneous under these conditions?
Moving to another question will save this response.
arrow_forward
Order: heparin sodium 20,000 units IV in 500 mL of 0.5 NS to infuse at 1,200
units/hour. What is the flow rate in mL/h?
(Enter numeric answer rounded to the nearest whole number.)
arrow_forward
Include all decimals #. Thank you
arrow_forward
SEE MORE QUESTIONS
Recommended textbooks for you

General, Organic, and Biological Chemistry
Chemistry
ISBN:9781285853918
Author:H. Stephen Stoker
Publisher:Cengage Learning

Organic And Biological Chemistry
Chemistry
ISBN:9781305081079
Author:STOKER, H. Stephen (howard Stephen)
Publisher:Cengage Learning,
Related Questions
- I Part 3 Discussion questions: Please answer in less than 2 paragraphs (20 points) 1. The FDA scientists have published 2 papers on the subject of sunscreen dermal absorption. What is different between the publications? The editorial page in the journal JAMA (Journal of American Medication Association) might help you answer this question.arrow_forwardThink about the situation where you are investigating the relationship of study time vs. exam grade.a. Which variable would be on the x-axis (and why)?b. Which variable would be on the y-axis (and why)?c. Would you expect the slope to be positive or negative (and Why)? 2) Contrast and compare interpolation with extrapolation. Which is “safer” to use and why?3) Look up how “the best fit straight line” is constructed. The term “Least squares” should be in your explanation.4) If I plot some data and end up with a r2 of (-) 0.99, is my plot useless? Explain.arrow_forwardPre-Lab Questions Experiment 12 Food Chromatography: The Distance Our Food Travels Name: Section: Instructor: Date: Imagine you have a solution that is comprised solely of component A. You have another solution that is comprised solely of component B, and yet a third solution that is comprised solely of component C. Component A's retention factor is about 0.84, Component B's retention factor is about 0.21, and Component C's retention factor is 0.55 in the same solvent system. You then have a fourth solution that is composed of components A and C. 145 You spot each solution on a piece of chromatography paper and allow the solutions to separate. Sketch what you think your paper would look like below.arrow_forward
- Question: Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected. My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC. -Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.arrow_forwardWhat can labs do to reduce error in GC- ms??arrow_forwardInstructions You are required to produce a short lab report using the information given in the accompanying document, ‘Lab report assessment_student data’. This document introduces an experiment, and also provides a set of experimental data for this experiment. You are required to use this data to perform steps 8 and 9 of the procedure. This includes calculations of a particular value, and then comparison to the true value. Consider any experimental errors when comparing calculated values and true values. Your report should be between 300 and 500 words (including all table contents and titles of tables and graphs). Your lab report should consist of three sections only: • Experimental • Results and Discussion • Conclusion You do NOT need to include an introduction section. Your experimental section should be written in appropriate lab report format, (passive voice, 3 rd person, in paragraphs). Your results and discussion section should contain the data presented in table format, and…arrow_forward
- There is one incorrect answer here. Can you help me figure out which one?arrow_forwardUsing the data provided please help me answer this question. Determine the concentration of the iron(Ill) salicylate in the unknown directly from to graph and from the best fit trend-line (least squares analysis) of the graph that yielded a straight line.arrow_forwardwhy is it importnant to write a lab report once the experiment is finishedarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- General, Organic, and Biological ChemistryChemistryISBN:9781285853918Author:H. Stephen StokerPublisher:Cengage LearningOrganic And Biological ChemistryChemistryISBN:9781305081079Author:STOKER, H. Stephen (howard Stephen)Publisher:Cengage Learning,

General, Organic, and Biological Chemistry
Chemistry
ISBN:9781285853918
Author:H. Stephen Stoker
Publisher:Cengage Learning

Organic And Biological Chemistry
Chemistry
ISBN:9781305081079
Author:STOKER, H. Stephen (howard Stephen)
Publisher:Cengage Learning,