PhysioEx Exercise 1 Activity 1

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West Coast University, Orange County *

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Chemistry

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Feb 20, 2024

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2/3/24, 11:43 AM PhysioEx Exercise 1 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/ 1/4 PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 1: Simulating Dialysis (Simple Diffusion) Name: Sophie Vanderkallen Date: 3 February 2024 Session ID: session-af9f4442-b726-8313-36d9-9a4bafebf8e4 Pre-lab Quiz Results You scored 100% by answering 3 out of 3 questions correctly. Experiment Results Predict Questions Stop & Think Questions The driving force for diffusion is You correctly answered: the kinetic energy of the molecules in motion. 1 In diffusion, molecules move You correctly answered: from high concentration to low concentration. 2 Which of the following dialysis membranes has the largest pore size? You correctly answered: 200 MWCO. 3 Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? You did not answer this question. 1 Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average molecular weight of a single amino acid is 135 g/mol. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer: both glucose and albumin. 2 The reason sodium chloride didn't diffuse left to right is that You did not answer this question. Correct answer: the membrane pore size was too small. 1
2/3/24, 11:43 AM PhysioEx Exercise 1 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/ 2/4 Experiment Data Solute MWCO Left Solute (mM) Right Solute (mM) Average Diffusion (mM/sec) Na Cl 20 9.00 0.00 0.0000 Urea 20 0.00 0.00 0.0000 Albumin 20 0.00 0.00 0.0000 Glucose 20 0.00 0.00 0.0000 Na Cl 20 0.00 0.00 0.0000 Urea 20 9.00 0.00 0.0000 Albumin 20 0.00 0.00 0.0000 Glucose 20 0.00 0.00 0.0000 Na Cl 50 9.00 0.00 0.0150 Urea 50 0.00 0.00 0.0000 Albumin 50 0.00 0.00 0.0000 Glucose 50 0.00 0.00 0.0000 Na Cl 50 18.00 0.00 0.0273 Urea 50 0.00 0.00 0.0000 Albumin 50 0.00 0.00 0.0000 Glucose 50 0.00 0.00 0.0000 Na Cl 100 9.00 0.00 0.0150 Urea 100 0.00 0.00 0.0000 Albumin 100 0.00 0.00 0.0000 Glucose 100 0.00 0.00 0.0000 Na Cl 100 0.00 0.00 0.0000 Urea 100 9.00 0.00 0.0094 Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mol. There is no reason to run these solutes at the 20 MWCO because You did not answer this question. Correct answer: glucose and albumin are both too large to pass. 2 The rate of diffusion for urea You did not answer this question. Correct answer: is slower than that for sodium because urea is a larger molecule. 3 + - + - + - + - + - + -
2/3/24, 11:43 AM PhysioEx Exercise 1 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/ 3/4 Albumin 100 0.00 0.00 0.0000 Glucose 100 0.00 0.00 0.0000 Na Cl 200 0.00 0.00 0.0000 Urea 200 0.00 0.00 0.0000 Albumin 200 0.00 0.00 0.0000 Glucose 200 9.00 0.00 0.0042 Na Cl 200 0.00 0.00 0.0000 Urea 200 0.00 0.00 0.0000 Albumin 200 9.00 0.00 0.0000 Glucose 200 0.00 0.00 0.0000 Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Review Sheet Results + - + - The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to You correctly answered: increase the rate of diffusion. 1 Describe the difference between the rate of diffusion seen for sodium and urea. You correctly answered: Urea diffused more slowly because it is larger than sodium. 2 Which of the following solutes did not pass through any of the membranes? You correctly answered: albumin. 3 When diffusion stops, we say the solution has reached You correctly answered: equilibrium. 4 Describe two variables that affect the rate of diffusion. Your answer: size of the molecule and the concentration gradient. 1 Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? Your answer: 2
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2/3/24, 11:43 AM PhysioEx Exercise 1 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex1/act1/ 4/4 I meant to put: No, not at all. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? Your answer: my answer was correct. 3 Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea. Your answer: Smallest: sodium chloride urea glucose albumin. 4