WORKSHEET 2

.docx

School

Egerton University *

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18

Subject

Chemistry

Date

Nov 24, 2024

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docx

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1

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1 NAME_____________________________________________________SECTION__________ CHEM455 BIOCHEMISTRY LABORATORY WORKSHEET 2.1 Determine DNA Concentration and Quality Sample # A260 A280 DNA concentration ( A = e l c) DNA sample quality A260/A280 1 0.211 0.111 0.527 (μg/mL) 1.901 2 3 4 Hint: 50 μg/mL of double stranded DNA has one (1) unit absorbance at 260 nm (i.e. A260 = 1). Answer the following questions: 1) Based on the concentration, calculate the amount of plasmid DNA (in μg) from your DNA extraction and purification experiment? Amount of plasmid DNA (μg) = Concentration (μg/mL) x Volume (ml) Concentration (μg/mL) = 0527 (μg/mL) Volume (mL) = 50 mL = 0527 (μg/mL)* . 50 ml = 26.4 μg 0) Based on the A260/A280 ratio, could you explain what contaminants does your DNA sample have? The ration is A260/A280 = 0.211/0.111 = 1.901 Based on this ratio of 1.9 is generally within the acceptable range for DNA purity. The typical range for pure DNA is considered to be between 1.8 and 2.0. In this case, a ratio of 1.9 indicates that the DNA sample is relatively pure and has minimal contamination.
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