Lab 6 Notes

From the spectrum, what color would the unknown solution appear to be?

2. You have two solutions of the same compound that are blue. One is very pale and the other is very intense. Absorbance of each solution was measured with a spectrophotometer at the same wavelength at which the solution absorbs. a) Which solution has the higher absorbance? b) Which is the more concentrated solution?

Why were the absorbance measurements recorded at lambda max (504 nm)?   Question 1 options:   at lambda max impurities in the sample do not absorb any light   Because the allura red dye absorbs red color at 504 nm   at lambda max the absorbance is the most sensitive to dye concentration   at lambda max the absorbance is the least sensitive to dye concentration

Please rank the wave length of maximum absorbance for each dye given below

Sample #1 has an absorbance of 0.300. Sample #2 is the same substance and has an absorbance of 0.600 using the same equipment. What is the relative concentration of the second sample? Select one: a. The concentration of sample #2 is the square root of that of #1. b. The concentration of sample #2 is that of sample #1 squared. c. The concentration of sample #2 is half that of sample #1. d. The concentration of sample #2 is twice that of sample #1.

Given the absorbance spectrum shown above for substance A in solution, what wavelength would you choose for absorbance/concentration studies for solutions of A?    A 470 nm  B  520 nm  C  585 nm D   600 nm E   650 nm

Test substance-A has a strong absorbance at 525nm (green light) but low absorbance at 730nm (red light). Substance-B has a very low absorbance of green light, but very high absorbance of red light. From this, it can be determined that... Select one: O a. None of these can be determined from the available data. O b. Substance A is fluórescent, but substance B is not. O c. Substance B contains contaminants. O' d. Substance A and substance B are the same material. e. Substance A and substance B are different materials. Clear my choice

You have the following solutions, A-E. Which solution has the highest absorbance value at 280 nm? Solution A: 1.0 mM Gly-Ser Solution B: 10 mM Gly-Ser Solution C: 1.0 mM Phe-Ser Solution D: 1.0 mM Tyr-Ser Solution E: 1.0 mM Trp-Ser Solution C Solution E Solution B Solution D Solution A

what do the peaks in a IR spectrum represent?

What wavelength(s) does methyl orange absorb the least? Include units. What color(s) does methyl orange absorb the least?

Rank these colors from the best to the least absorber of radiation. (Best absorber - I; Least Absorber - IV) WHITE GREEN BLACK  BLUE

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To make a measurement of the Absorbance of a solution, which of the following must be known # A. The molar extinction co-efficient of the compound B. The concentration of the solution C. The chemical formula for the compound D. A wavelength of light at which the compound absorbs light

Beside each spectrum, list the important IR absorbances and the type of bond causing each signal. Draw the structure that produced each spectrum inside the spectrum. Br HO LO0 D 4000 3000 2000 1500 1000 HAVENUMBERI -il

In this structure, which hydrogen has the higher chemical shift and why?

Select everything that applies to the IR spectrum below 0.8 0.6 0.4 0.2 0.0 3000 2000 Wavenumber (cm-1) The spectrum shows signal(s) >3000 The spectrum shows a characteristic signal ~2200 The spectrum shows a characteristic signal ~ 1700 The spectrum shows a signal characteristic of OH The spectrum shows a signal characteristic of C=C/C=N The spectrum shows a signal characteristic of C=O The spectrum shows a signal characteristic of H-C= The spectrum shows signal(s) characteristic of H-C= O-C-OH is likely present in the compound giving this spectrum 1000

Q1

Why does Cytochrome C have a peak absorbance of 409nm? When proteins usually have a peak absorbance of 280nm..?

find the compounds based of the picture  -label the proton spectrum on the graph, please  -label carbon spectrum on the graph  * indicate the labels on the diagram correlating to the structure

Beside each spectrum, list the important IR absorbances and the type of bond causing each signal. Draw the structure that produced each spectrum inside the spectrum. OH Br L00 4000 3000 2000 1500 1000 HAVENUMBERI -1l TRENSMETTENCEI

Which one of the following atomic spectroscopy methods gives the smallest detection limit for sodium? Flame Absorption Electrothermal Absorption Flame Emission ICP torch Emission

Choose the compound with the most intense IR signal at -1700 cm1. CI .CI .CI CI A B D

Chemistry 1. Expected Signals ('H NMR and ¹3C NMR) TEUERSutet In the boxes provided, indicate the number of signals that would be expected in the 'H NMR and 13C NMR 13C NMR ¹H NMR 13C NMR ¹H NMR 13C NMR ¹H NMR HOT H-N 13C NMR 1H NMR 13C NMR ¹H NMR 13C NMR ¹H NMR

The absorbance of a sample as measured by UV-visible spectrometry is inversely proportional to the concentration of the sample, such that, as the concentration increases, the absorbance decreases.   True  False#

The picture of the question is attached

2) From here, the student wants to narrow down Unknown 1 and 2 even further. Fill in the table below to help identify the unknowns. Note: By visual inspection, Unknown 1 is a yellow solid and Unknown 2 is a white color. Compound Absorbance Frequency Absorbance Wavelength Absorbed Color Fluorene 300 nm Ultraviolet 7.14x1014 s-l 8.33x1014 s- 7.5x1014 s-l Fluorenone Fluorenol Ultraviolet 4-Nitrophenol Coumarin 280 nm a) Based on the table above and what you learned in Q1 what is the identity of Unknown 1? b) Based on the table above and what you learned in Q1 what is the identity of Unknown 2?

100 1000 S80 3000 2000 DOST 4800 NAVENUMBERI-l Functional group Critical absorbance frequency(ies), cm1 Identity: