Lab 6 Notes

E) 1. What is Amax for this compound? 2. What light would you use to analyze this sample? What color is absorbed and what color is transmitted by the sample? 500 700 vamiengthin anctes (aen) apoy ga

2. You have two solutions of the same compound that are blue. One is very pale and the other is very intense. Absorbance of each solution was measured with a spectrophotometer at the same wavelength at which the solution absorbs. a) Which solution has the higher absorbance? b) Which is the more concentrated solution?

Predict what a graph of absorbance versus concentration would look like. Sketch your prediction.

Given the absorbance spectrum shown above for substance A in solution, what wavelength would you choose for absorbance/concentration studies for solutions of A?    A 470 nm  B  520 nm  C  585 nm D   600 nm E   650 nm

Using the following data, if an unknown solution of Orange Dye X had a measured absorbance of 0.345, what is the concentration Orange Dye X in the solution?  Concentration (mM)  Absorbance 0.005 0.143 0.010 0.285 0.015 0.428 0.020 0.570

3. What is the purpose of using blank before measuring the absorbance of a substance?

How would you calculate Ɛ from the gradient of an absorbance vs concentration plot?

Rank these colors from the best to the least absorber of radiation. (Best absorber - I; Least Absorber - IV) WHITE GREEN BLACK  BLUE

please please answer super super fast i really need it its very urgent and important please answer everything i need it before the end of today before 11:59pm I would really appreciate it if you would answer fast

1) of a solution appears green, what color of light is most likely to absorbed the strongest?

1. What color does sunlight appear to the naked eye without passing through a prism, diffraction grating. or spectrometer?

ge 1: 4 ! Q A 2 5 -- 3 :0 F1 @ 2 30% F2 # 3 Based on the following spectrum, what color would you expect this solution to appear? WE 1.8 1.6 Absorbance 1.4 1.2 1 0.8 0.6 0.4 0.2 0 380 Trouble viewing? Oa) red Ob) yellow 80 F3 O d) purple c) orange e) blue $ 4 430 Q F4 % сч оро 5 480 0 F5 R T A 6 530 580 Wavelength (nm) F6 Y & 7 K F7 630 nm- Orange 590 nm 630 U * Red Yellow 8 F8 750 nm 400 nm 680 560 nm I ( 9 F9 S D F G H J K Violet Green ) 0 430 nm 480 nm Blue F10 0 L P F11 Ć + { [

What is the color of a KMnO4 solution? Which solution, 0.50M or 0.25M, will be darker in tone? 2. What is the corresponding absorption to a transmission level of 63%? 3. If a solution has 0% transmission, what does that tell about the solution and the amount of light it absorbs? 4. What is the molarity of a solution containing 40g of ZnCl2 dissolved in 1000mL of solution?

a solution has a %T value of 51.6% at 550 nm. What us the absorbance of this solution at this wavelenght?

Why does Cytochrome C have a peak absorbance of 409nm? When proteins usually have a peak absorbance of 280nm..?

do they absorb light between 200-400nm?