cellular resp worksheet

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Jan 9, 2024

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Cellular Respiration Worksheet BSCI170, Fall 2023 1. Consider the diagram below of the mitochondrial electron transport chain. a) Label all 7 components (molecules/proteins/complexes) (abbreviations work!). b) Name the compartment on each side of the membrane, and identify which membrane is shown. c) At the correct arrows, write the substrates and products of the oxidation reaction of the high energy electron donors. d) Indicate the path electrons flow through this ETC. e) At the correct arrow, write the reaction (substrate and product) for the reduction of the final electron acceptor. f) Which components are proton pumps? C1, C2, C3, C4 g) In which compartment will there be a high H+ concentration? Intermembrane h) In which protein/complex chemiosmosis takes place? ATP synthase i) In which compartment will ATP be made? matrix Item Location Item Location Pyruvate C Plastoquinone (PQ) F NADPH E Ubiquinone (Q) J One place with ATP synthase F Another place with ATP synthase J 2. Referring to the plant cell diagram below, match each item with an appropriate location. C1 Compartment? Membrane? Compartment?
3. You are expected to know the substrates and products for each pathway in cellular respiration as well as how the connect together. Note: For substrates, by convention we will ignore "recyclables" and assume there is normally a pool of those available. Theses include: ADP, Pi, NAD+, FAD, H+, and O 2 . For products, on the other hand, we include everything because they are all important for the cell. Complete the following table: Pathway Substrates Products (include numbers) Glycolysis Glucose 2 pyruvate, 2 ATP, 2 NADH Transition step (pyruvate oxidation) 2 pyruvate 2 acetyl-coA, 2 C02, 2 NADH Citric acid cycle 2 acetyl-CoA 2 ATP, 6 NADH, 4 CO2, 2 FADH2 Oxidative phosphorylation (ETC + chemiosmosis) 10 NADH, 2 FADH2 34 ATP 4. You will not be responsible for all the details of the pathways (you should be able to look them up if needed), but there are some important features you are expected to recognize: a) Starting from one glucose, how many ATP are made by substrate level phosphorylation ? Which pathway(s) are making those ATP? 4 ATP, glycolysis and the citric acid cycle b) Starting from one glucose, how many ATP are made by oxidative phosphorylation ? Which pathway(s) are making those ATP? 34 ATP by chemiosmosis and mitochondrial ETC. c) We talk about glycolysis as having two phases - what are they called? For each one, explain what is happening to ATP, and how that relates to the net gain in ATP at the end of glycolysis. Investment and payoff phases. In the investment phase, 2 ATP is used to break down glucose, and in the payoff phase, two ATP is made. The amount of ATP in glycolysis is 4, but since 2 was used to break down glucose in the investment phase, the net gain is 2.
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