gene expression worksheet

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University of Maryland, College Park *

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170

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Biology

Date

Jan 9, 2024

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pdf

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Gene Expression Worksheet BSCI170, Fall 2023 Part I. Structure-Function Relationships in Genes Consider the "two-line model" of a gene shown below - each line represents one strand of a DNA double helix, and the transcription start site is indicated as +1 . 1. Assume that you know RNA polymerase will move to the right during transcription. a. Is upstream to the left or right of +1? b. Is the promoter to the left or right of +1? c. Is the TATA box to the left or right of +1? d. Is the transcribed region to the left or right of +1? e. Which strand is the template , top or bottom? 2. Now, let's try that again! This time assume that you know RNA polymerase will move to the left during transcription. a. Is upstream to the left or right of +1? b. Is the promoter to the left or right of +1? c. Is the TATA box to the left or right of +1? d. Is the transcribed region to the left or right of +1? e. Which strand is the template , top or bottom? 3. In your own words, describe how you determined on which side of +1 the RNA transcript will be made, and how you determined the template strand?

Part III. Gene Expression Practice Consider the following sequence from one stand of DNA in a eukaryotic gene: 5'-GCGTATAATTCGACCGTGAATTACATAT G AACGCAGTTATGATTCCATGTGCTTTTTCGGGTTATAGAATC-3' 3'-CGCATATTAAGCTGGCACTTAATGTATA C TTGCGTCAATACTAAGGTACACGAAAAAGCCCAATATCTTAG-3' 7. Is upstream to the left or right of +1? How did you decide where this should be? 8. What RNA that will be transcribed? Write out the sequence and label the ends (Just go all the way to the end as not terminator is given). 9. What polypeptide will be made from this mRNA? +1 -25

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