Lab 04 Data- finished

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Jan 9, 2024

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Lab 04 Data Name: Raven Chalmers 1. Visualizing the reaction of catalase : Catalase is an enzyme that converts hydrogen peroxide (2H 2 O 2 ) into water (2H 2 O) and oxygen (O 2 ). When a gas such as oxygen is release into a liquid, like water or hydrogen peroxide, it will appear as bubbles. Enzymes are proteins. a. How many mL of bubbling (just the bubbles) did you observe in the “C” tube of water and potato? 6mLs b. Was catalase producing oxygen in the C tube? If not, why not? Yes it was, there were bubbles indicating that there was oxygen being produced c. How many mL of bubbling did you observe in the “T” tube of hydrogen peroxide and potato? 0mLs d. Was catalase producing oxygen in the T tube? If not, why not? No, there was no reaction (bubbling) which would have indicated the production of oxygen e. Insert an image of your two control tubes (tubes C and T) from the end of the experiment.
2. The effect of pH on catalase a. How many mL of bubbling (just the bubbles) did you observe in the “A” tube of vinegar, potato, and hydrogen peroxide? 0mL b. How many mL of bubbling (just the bubbles) did you observe in the “N” tube of water, potato, and hydrogen peroxide? 1mL c. How many mL of bubbling (just the bubbles) did you observe in the “B” tube of baking soda, potato, and hydrogen peroxide? >1mL d. Which tube had the most bubbling (A, N, or B)? N e. What was the general pH of this tube (acidic, neutral, or basic – these were tested in a previous lab!) Neutral f. Why did the other tubes have less bubbling? Be specific – use what you know about pH and enzymes/proteins. 3% Hydrogen peroxide has pH of 6.5 so it’s pretty acidic. When you put an acid in with another acid (tube A) you wouldn’t really expect much of a reaction, they’d cancel each other out. In the B tube, the 2% sodium bicarbonate has a pH of 8.5, it’s slightly basic. (I’m not sure about this response, I
struggled here)-> The solution itself already has a fair part of water within it, but when the enzyme goes to work and attempts to convert the water to oxygen gas, the baking soda cancels out the acidity, and therefore less bubbles form compared to tube N. g. Insert an image of your three pH tubes (tubes A, N, B) from the end of the experiment.
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Plant data: By now you should have at least one seedling sprouting, either from the very first lab, or after following the “plant germination remediation” instructions in week 3 3. What percentage of seeds from cup 1 sprouted (find the percent by taking the number of sprouted seeds divided by the total seeds, then multiplying it by 100)? 25% 4. What is the height of the tallest sprout in cm? (If there is only one sprout, that would be the tallest – if there are no sprouts, write, “no sprouts”) 2cm 5. What percentage of seeds from cup 2 sprouted (find the percent by taking the number of sprouted seeds divided by the total seeds, then multiplying it by 100)? 20% 6. What is the height of the tallest sprout in cm? (If there is only one sprout, that would be the tallest - if there are no sprouts, write, “no sprouts”) 1.5cm
7. Please insert a photo of both your plant cups here – make sure each is clearly labeled (e.g. cup 1 and cup 2): Please note: You will need your plant data throughout the semester, so be sure to keep a copy of this information – you may want to think of how this particular lab relates to your plant data…
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