PHM633PlateQuiz

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Seneca College *

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633

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Biology

Date

Jan 9, 2024

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pptx

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14

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Media Demonstration
Question 1: Which of the following show variable growth. • A) Soybean Casein Digest agar • B) Sabouraud Dextrose agar • C) Plate Count agar • D) All are correct • E) None apply
Question 2: Trypticase Soy Agar has the following function(s): • A) Used to obtain a pure culture • B) Used to maintain stock cultures • C) Used to obtain total microbial plate counts • D) Used to obtain a nutrient-rich environment, supporting the routine of growth of a wide variety of microorganisms.
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Question 3: Which media contains neutral red pH indicator which aids in the visual differentiation of microbes that grow on this media • A) MacConkey agar • B) Eosin Methylene Blue agar • C) Mannitol Salt agar • D) Violet Red Bile agar • E) Brilliant Green agar
Question 4: Which of the following statement(s) is true for BPA or MSA. Select all that apply. • A) Inhibit the growth of gram negative organisms (True for both MSA and BPA) • B) Differential media for Staphylococci (True for MSA and BPA) • C) Selective media for Staphylococci (True for BPA and MSA) • D) Contains Sodium Pyruvate to simulate growth of Staphylococci (True for BPA) • E) S.aureus appears as yellow colonies with yellow zones (True for MSA)
Question 5: Which of the following media help with the Identification of Salmonella. Select all that apply. • A) Xylose-Lysine-Deoxycholate Agar (XLD) • B) Eosin Methylene Blue Agar • C) Brilliant Green Agar • D) Any other agar is wrong except for Salmonella-Shigella agar and Bismuth Sulfite agar
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Question 6: This media has a low pH and high sugar content which is mildly selective for bacteria. Select all that apply • A) Sabouraud’s Dextrose agar (SDA) • B) Nutrient Agar • C) Xylose-Lysine-Deoxycholate agar (XLD) • D) Tryptic Soy Agar
Question 7: Match the following media to their respective colony descriptions for certain bacteria. • Cetrimide (CET) agar: Blue-green colonies with blue-green halo in surrounding media • EMB agar: Dark Purple colonies possibly with a metallic green sheen • BPA agar: Grey-Black colonies and can have a metallic silver shine • Bismuth Sulfite (BS) agar: Grey-Black colonies and can have a metallic silver shine • MSA agar: Yellow convex with circular colonies with yellow zones
Question 8: Dipotassium phosphate and Magnesium sulfate stimulate pyocyanin production. • True • False Note: Dipotassium phosphate simulates fluorescein production
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Question 9: Cetrimide agar helps in the identification of Pseudomonas aeruginosa. • True • False
Question 10: High sugar concentration and low pH in Potato Dextrose agar acts as a bacterial inhibitor. • True • False
Question 11: Baird Parker Agar contains ferrous sulfate that reacts with hydrogen produced by Salmonella creating a black colour. • True • False Note: Bismuth Sulfite Agar reacts in this manner not Baird Parker Agar.
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Question 12: Crytal Violet and Bile salts interfere with the formation of the peptidoglycan layer of gram positive organisms on MacConkey agar. • True • False Note: This is true. These agents are the selective component of the media and will inhibit gram positive microbes from growing but allow for gram negative microbes to grow.
Question 13: BSA uses 7.5% sodium chloride to inhibit gram negative organisms from growing allowing for the identification of S.aureus. • True • False

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