BIO 101 Photsyn-Lab-Handout

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Jan 9, 2024

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Exploring Photosynthesis and Plant Pigments Darnell Lawrence 01/03/2024
Data Activity 1: Plant Pigments Overview Data Table 1: Chromatography of Plant Pigments Band # Distance from origin (mm) Band Color/ Identification 1 16 mm Dark Green 2 17 mm Green 3 23 mm Light Green 4 25 mm Lime green Solvent front 51 mm Clear 1. Many things can affect the evaporation of solvents during a chromatography experiment. To allow researchers to compare results across experiments under different conditions, scientists use an R f value, that is the ratio of the distance the pigment traveled to the distance the solvent traveled. This number remains constant across trials. Calculate the R f value for each pigment. Band one 16/51= 0.3 mm Band two 17/51=0.3mm Band three 23/51=0.4mm Band four 25/51=.5mm Activity 2: Leaf Disk Assay Overview 2. Write a hypothesis that the Leaf Disk Assay is designed to test.
If leaf disks are exposed to light, they will be able to photosynthesize, while the leaf disks that are not exposed to light (kept in the dark) will not be able to photosynthesize. Data Table 2: Leaf Disk Assay, Light Treatment Time (minute s) A (0.24% ) B (0.24% ) Average of A and B A (0.12% ) B (0.12% ) Average of A and B 0 0 0 0 0 0 0 5 0 0 0 0 0 0 10 3 3 3 0 0 0 15 4 5 4.5 2 1 1.5 20 8 8 8 3 1 2 25 8 10 9 4 3 3.5 30 10 10 10 4 4 4 35 10 10 10 4 4 4 40 10 10 10 4 6 5 45 10 10 10 7 7 7 50 10 10 10 8 8 8 55 10 10 10 10 10 7 60 10 10 10 10 10 7.5 Data Table 3: Leaf Disk Assay, Dark Treatment Time (minute s) A (0.24% ) B (0.24% ) Average of A and B A (0.12% ) B (0.12% ) Average of A and B 0 0 0 0 0 0 0 5 0 0 0 0 0 0
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10 0 0 0 0 0 0 15 0 0 0 0 0 0 20 0 0 0 0 0 0 25 0 0 0 0 0 0 30 0 0 0 0 0 0 35 0 0 0 0 0 0 40 0 0 0 0 0 0 45 0 0 0 0 0 0 50 0 0 0 0 0 0 55 0 0 0 0 0 0 60 0 0 0 0 0 0 3. Describe the experimental controls used in the Leaf Disk Assay experiment(s). The volume of solution in each syringe (6 ml) The light source (Desk lamp) The type of plant leaves (spinach) The size of leaf disks Temperature of room Time spent in solution and data recording intervals. 4. Describe the variable(s) used in the Leaf Disk Assay experiment(s). Independent variable: light intensity Dependent variable: the rate of photosynthesis Controlled variables: the size of the plant disks, the volume of water used, and its temperature, the amount of baking soda used
5. Compare the test groups of the Leaf Disk Assay. Which treatment (syringe) had the most floating leaf disks after 35 minutes? After 35 minutes the syringes in the light environment had more floating leaf disks than the syringes in the dark. More specifically, the syringes containing 0.12% concentration produced more floating disks than that of the 0.24% solution. 6. Were there any treatments (syringes) without floating disks? Yes, the ones with the dark treatments had no floating disks. 7. How do floating disks correspond to the rate of photosynthesis? Floating disks correspond to the rate of photosynthesis because when the disks are floating, they are exposed to more light, which means they can photosynthesize more efficiently. The disks are also able to move around, so they can follow the sun and maximize their exposure to light. 8. According to your data, does light intensity affect the rate of photosynthesis? Explain. Light most definitely affects the rate of photosynthesis, the energy from light is critical to the entire process, without it, it could not take place. The data clearly illustrates this because as time goes on for the light treatment, the amount of floating leaf disks consistently increases, and for
the dark treatment, the disks do not float for the entirety of the experiment. 9. How did the baking soda solution affect photosynthetic rates? The higher the concentration of baking soda, the more the rate of photosynthesis increases. Photosynthesis requires water, and carbon dioxide to produce glucose and oxygen as their by- product- H 2 O + CO 2 <=> C 6 H 12 O 6 + 6 O 2 Thus, the more reactants on the left side, the more products on the right side. Sodium bicarbonate (baking soda) when mixed in water produces the by-product bicarbonate and sodium ions. Bicarbonate (HCO 3 ) would then be the new source of carbon in photosynthesis; thus it increases the number of reactants on the left. Graph
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