Brittany Payne
The Effect of Temperature on Enzyme Function
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Methods
This experiment required test tubes, test tube rack, catalase dropper, hydrogen peroxide, glass rod, heat starter, and thermometer. Adding 4 mL of hydrogen peroxide to a test tube, and measure the volume of bubbles, this will be the controlled group of the experiment. In the second test tube, add 1 mL of catalase, measure temperature of catalase in the tube, then add 4 mL of hydrogen peroxide, stir the solutions in the tube and measure the volume of bubbles at 20 seconds. Continue for every temperature requested.
Enzymes are a substance produced by a living organism which acts as a catalyst to bring about specific biochemical reaction. Catalyst is a substance that increases the rate of a chemical reaction without itself undergoing any permanent chemical change. A test tube will contain a catalase and hydrogen peroxide which will be the experimental group that is being manipulated; the other test tube will contain the control group (volume of bubbles 1). Saying this, my hypothesis is as the temperature increases so will the reaction rate up to a point. Introduction
Results
Conclusions
After conducting a series of experiments, the experiment suggests that the hypothesis is supported. By the results gathered from the experiment, it was evident as the temperature increases, the reaction rate will increase also up to a certain point.
This experiment showed how “molecules will more faster at higher temperature rates” (1). The “faster movement of molecules increase the chance of collision, thus making the binding between substrate and enzyme more likely” (1). It is important to understand that using catalysts will lead to faster, more energy-efficient chemical reactions. Limitations and Assumptions
Biological Principles 1 Laboratory,, Kennesaw State University
Figure 1: Bubbles over Temperature
Figure 1: Line graph represents the volume of bubbles in the container over a certain degree of temperature in °C
Table 1: Catalase Temperature (°C) Vs Volume of Bubbles (mL)
Results text: At the beginning of the experiment, both test tubes have the same amount of hydrogen peroxide. After adding 1mL of catalase in one test tube at the different temperatures, the volume of bubbles changes constantly. At 25 °C, the volume of bubbles in the container one is 1.5 whereas the volume of bubble in container two is 2. Once the temperature is at 37° C, there will be a decrease in volume of bubbles. While the controlled group had close numbers on both test tubes, the tube with catalases increase volume more than the tube without it. (Table 1) Hence, once a certain temperature is met, the volume of bubbles will then decrease. (Figure 1)
Table 1: The volume of bubbles in the containers over a certain degree of temperature in °C
References
Due to this experiment only being recorded every 20 seconds, any minute longer would decrease the amount of volume in the test tube. Not being careful to only add 1 mL of catalase in the tube, adding anything over the desired amount would change the experiment entirely. 1. McGraw Hill, Connect 3P Inclusive Access Online
Access for Biology Virtual Labs, 1st ed. (2021) Lab 6 How Enzymes Function: Effect of Temperature
2. Urry, L. A., Cain, M. L. 1., Wasserman, S. A., Minorsky, P. V., Reece, J. B., & Campbell, N. A. (2017). Essential biology
. Eleventh edition. New York, NY: Pearson Education, Inc.
Temperature °C
Volume of Bubbles 1
Volume of Bubbles 2
0
0.5
0.5
25
1.5
2
37
5
4
45
3
3
55
1.5
65
0
State Hypothesis
Identify scenarios
Test the Hypothesis
Execute tests and allow one variable to be manipulated
Record Data
State the conclusion. Did the hypothesis fail or support?
