BIOL 3053 Lab 1 Assignment (Winter 2022)

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Memorial University of Newfoundland *

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3053

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Biology

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Dec 6, 2023

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docx

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Memorial University of Newfoundland Department of Biology Biology 3053 Lab 1 Assignment Safety Procedures and Precautions, Cultivation Procedures and Aseptic Techniques Winter 2022 Name: Julie Hollahan Student #: 201311107 Exercises 1: Cultivation Procedures Results and Observations: (3.0 marks) Medium Describe growth* Was there any contamination?** (y/n) Likely source of contamination (a) TSA broth Broth was cloudy with white turbid at the bottom of tube. Once the tube was shaken this turbid disappeared and no growth was noted. N N/A (b) TSA slant A transparent, almost white in color noted during observation. Specimen noted to be irregular in shape, flat form and undulate margins noted along the slant. N N/A 1

(c) TSA plate Punctiform in shape, convex form noted, margins are entire. Colony measuring ata ~0.2cm N N/A 1. If you were instructed to swab the total surface of the plate, how could you ensure that you get total coverage the next time? (0.5 mark) I would ensure total coverage by swabbing the plate in a back and forth motion across the entire plate, ensuring that there are no gaps in between each swab. I would also use both sides of the swab, ensuring good contact between the swab and plate. 2. Why is it important that broth media be perfectly clear? (0.5 mark) It is important that broth media be perfectly clear in order to see if a culture is growing or not. 3. What is the purpose of agar plates? (0.5 mark) Agar plates ensure a solid foundation in which bacteria can grow. Exercises 2: Aseptic Techniques 1. This Exercise dealt with the Aseptic Technique, describe 4 instances where your knowledge and practice of the Aseptic Technique would be important to the health of your patient. (1.0 mark) During a procedure/skill, prior to surgery, post-op and discharge instructions (ie. dressing changes at home) 2. When sterilizing the loop, why do you need to insert the loop with organisms first in the coolest part of the flame? (0.5 mark) This prevents microorganisms that are still living exponentially growing, then “splashing” and contaminating near surfaces. 3. Explain the purpose of each of the following procedures and how they prevent contamination. (a) incubating an inoculated Petri dish in the inverted position. (1.0 mark) An inoculated petri dish must be incubated in the inverted position due to water droplets forming on the lid of the petri dish. Inverting the dish removes the possibility of contaminating the agar medium and disrupting the growth of the specimen. (b) flaming the mouth of the culture tube after opening and before closing it. (1.0 mark) This decreases the likelihood of bacteria or foreign material from entering the tube, as it causes an outward flow of air. 4. How each of the following prevents contamination: 2

(a) Petri dish? (1.0 mark) Petri dishes prevent contamination by having a loose fitting lid that dust and other foreign materials are unable to pass through, there is a small space for air to flow through, but gravity prevents any particles from entering. (b) Cotton-plugged culture tube? (1.0 mark) The cotton acts almost like a spider web, trapping any microbes that may enter the tube, but allows for airflow for aerobic cultures to grow. 3

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