Electrophoresis+Lab+Student+Handout_rev

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Liberty University *

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DNA

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Biology

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Feb 20, 2024

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pdf

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Uploaded by UltraDangerWren26

The Electrophoresis Lab Worksheet Student Name: _____________________________________ Date: __________ Directions: Use the information in the “Electrophoresis Lab” lesson to help you complete this handout. 1. What is being measured using gel electrophoresis? (5 pts) 2. What molecules, other than DNA, is gel electrophoresis helpful for separating? (5 pts) 3. What causes the DNA sample to move in the electrophoresis setup? ( 5 pts) 4. Why is the DNA stained during the process of gel electrophoresis? (5 pts) 5. List the 5 steps of g el electrophoresis . (10 pts) 6. Why do the strands of DNA separate from one another when subjected to electrophoresis? How can we tell them apart? (10 pts) 7. Where are the DNA samples placed in the experimental chamber? ( 5 pts) 8. Wh y is a "DNA size standard" used? What does this do in the experiment? (5 pts)

9. When running a gel , the black corded end generate s a ________________ charge, and the red corded end generate s a ______________________ charge. (8 pts) 10. Which cord must you put closest to the wells to move the DNA through the gel? (5 pts) 11. When the power is turned on, bubbles can be seen in the chamber. What does this indicate? What is happening to the samples? (9 pts) 12. Which DNA strands move through the gel the fastest? Why? ( 8 pts) 13. Wh y is the gel stained and what stain is used? (5 pts) 14. Record the three DNA fragment length estimates from the gel below . (5 pts) 15. Refer to the picture labeled " DNA Fingerprinting” in the lesson. Which DNA sample from the children (labeled C1, C2, C3, and C4) has a DNA pattern most closely resembling the father's sample (F)? (10 pts)

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