Name: ________________________________________
Enzyme Lab - Virtual
Site: https://glencoe.mheducation.com/sites/dl/free/0078802849/383930/BL_11.html
Overview:
In this investigation, you will determine the effects of
substrate concentration and pH on the rate of an enzyme-catalzyed
reation.
Background: Amylase is
an enzyme that catalyses the hydrolysis of starch into sugars. It is
present in the saliva of humans and some other mammals, where it
begins the chemical process of digestion. Foods that contain large
amounts of starch, like bread, may acquire a slightly sweet taste as
they are chewed because amylase degrades that starch into sugar.
In the reaction shown, the enzyme is the amylase and the substrate is starch. The program measures the amount of produce produced after the reaction has progressed for 1 minute, allowing you to compare rates of reaction by looking at the amount of end product.
Procecure:
The simulation allows you to change two variables: pH level and the amount of available substrate. Data from the simulation can be viewed by clicking on the computer monitor.
*The simulation allows you to input data and print, but due to technical issues with browsers and networked computers, it is preferable to copy the data onto this page.
Data:
Collect data for each of the variables and complete the table below. The data collected is "number of molecules of product formed per minute."
Amount of Substrate
pH 3
pH 5
pH 7
pH 9
pH 11
0.5g
1.0g
2.0g
4.0g
8.0g
Analysis:
1.
Use a graphing program like Excel or "Google sheets" to graph the data showing 5 separate lines for each pH level. Attach this graph to this worksheet when you turn it in. To get started, you will be copying the data on the table above to your spread sheet.
