MICROBIOLOGY FUND.:CLINICAL APPR-ACCESS
MICROBIOLOGY FUND.:CLINICAL APPR-ACCESS
3rd Edition
ISBN: 2810022612789
Author: Cowan
Publisher: MCG
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Chapter 9.3, Problem 2MM

Q. What is the mode of action of chlorhexidine?

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When beta-lactamase was isolated from Staphylcoccus aureus and treated with a phosphorylating agent, only the active site, serine was phosphorylated. Additionally, the serine was found to constitute 0.35% (by weight) of this beta-lactamase enzyme. Using this, calculate the molecular weight of this enzyme and estimate the number of amino acids present in the polypeptide.
Based on your results from the Mannitol Salt Agar (MSA) media, which of your bacteria were mannitol fermenters and which were not mannitol fermenters?
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