Lehninger Principles of Biochemistry
7th Edition
ISBN: 9781464126116
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
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Chapter 9, Problem 9P
Summary Introduction
To determine: The reason behind using two antibodies successively for detection of a protein and the reason why one is labeled and why not using only one antibody.
Concept introduction:
Immunofluorescence is a microscopic technique used for identification of proteins by exploiting its antigenic property. This property is based on protein-protein interaction. The compound used for observation is a fluorophore bounded to an antibody which is specific to the antigen.
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