Lehninger Principles of Biochemistry
7th Edition
ISBN: 9781464126116
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
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Chapter 9, Problem 2P
Summary Introduction
To determine: How to identify recombinant yeast artificial chromosome (YAC) from other plasmid.
Introduction:
A vector is a DNA molecule which is used as a vehicle to transport foreign genetic material into the target cell. In target cell, it can be replicated and get expressed. A vector that contains a foreign DNA is termed as a recombinant DNA. The four classes of vectors are plasmids, cosmids, viral vectors, and artificial chromosomes.
DNA cloning is a technique which is used to make genetically identical copies of a gene or segment of DNA. Recombinant DNA technology allows the cloning of DNA to make recombinants. Vector such as plasmid used for cloning purposes. The desired gene can be inserted into the bacterium (host cell).
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1
2
Br
OCH₁₂
CF3
3
4
2. Which one shows the correct order of reactivity of these four compounds in
electrophilic aromatic substitution reactions, with more reactive > least reactive ?
A) 1>2>3>4
B) 13>4> 2
C) 3 > 2 > 1 > 4 D) 4 > 3 > 1 > 2 E) 3 >1>2> 4
12. Which of the compounds
below is expected to be the
major product formed by
this reaction sequence?
COOH
Br
A
C₂H5CI
H₂CrO4
Br₂
FeBr3
AICI 3
.COOH
.COOH
COOH
CH₂OH
B
Br
Br
C
D
Br
E
Br
11. Which of these chlorobenzenes does not undergo nucleophilic aromatic
substitution upon treatment with NaNH2?
CI
CI
CI
CI
CI
A
B
C
D
E
Chapter 9 Solutions
Lehninger Principles of Biochemistry
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