Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 9, Problem 8P
The linear bacteriophage λ genomic DNA has at each end a single-strand extension of 20 bases. (These are sticky ends but are not, in this case, produced by restriction enzyme digestion.) These sticky ends can be ligated to form a circular piece of λ DNA. In a series of separate tubes, either the linear or circular forms of the DNA are digested to completion with EcoRI, BamHI, or a mixture of the two enzymes. The results are shown here.
| a. | Which of a. the samples (A or B) represents the circular form of the DNA molecule? How do you know? |
| b. | What is the total length of the linear form of the λ DNA molecule? |
| c. | What is the total length of the circular form of the λ DNA molecule? |
| d. | Draw diagrams of the circular and linear λ DNA molecules, showing the locations of the EcoRI and BamHI sites. |
Expert Solution & Answer
Trending nowThis is a popular solution!
Students have asked these similar questions
If you had an unknown microbe, what steps would you take to determine what type of microbe (e.g., fungi, bacteria, virus) it is? Are there particular characteristics you would search for? Explain.
avorite Contact
avorite Contact
favorite Contact
୫
Recant Contacts
Keypad
Messages
Pairing
ง
107.5
NE
Controls
Media Apps Radio
Nav Phone
SCREEN
OFF
Safari File Edit View History Bookmarks Window Help
newconnect.mheducation.com
M Sign in...
S The Im...
QFri May 9 9:23 PM
w The Im...
My first....
Topic:
Mi Kimberl
M Yeast F
Connection lost! You are not connected to internet
Sigh in...
Sign in...
The Im...
S Workin...
The Im.
INTRODUCTION
LABORATORY SIMULATION
Tube 1
Fructose)
esc
- X
Tube 2
(Glucose)
Tube 3
(Sucrose)
Tube 4
(Starch)
Tube 5
(Water)
CO₂ Bubble Height (mm)
How to Measure
92
3
5
6
METHODS
RESET
#3
W
E
80
A
S
D
9
02
1
2
3
5
2
MY NOTES
LAB DATA
SHOW LABELS
%
5
T
M dtv
96
J:
ப
27
כ
00
alt
A
DII
FB
G
H
J
K
PHASE 4:
Measure gas bubble
Complete the following steps:
Select ruler and place next to tube
1. Measure starting height of gas
bubble in respirometer 1. Record in
Lab Data
Repeat measurement for tubes 2-5
by selecting ruler and move next to
each tube. Record each in Lab
Data…
Ch.23
How is Salmonella able to cross from the intestines into the blood?
A. it is so small that it can squeeze between intestinal cells
B. it secretes a toxin that induces its uptake into intestinal epithelial cells
C. it secretes enzymes that create perforations in the intestine
D. it can get into the blood only if the bacteria are deposited directly there, that is, through a puncture
—
Which virus is associated with liver cancer?
A. hepatitis A
B. hepatitis B
C. hepatitis C
D. both hepatitis B and C
—
explain your answer thoroughly
Chapter 9 Solutions
Genetics: From Genes to Genomes
Ch. 9 - Match each of the terms in the left column to the...Ch. 9 - For each of the restriction enzymes listed below:...Ch. 9 - The calculations of the average restriction...Ch. 9 - The DNA molecule whose entire sequence follows is...Ch. 9 - Why do longer DNA molecules move more slowly than...Ch. 9 - Agarose gels with different average pore sizes are...Ch. 9 - The following picture shows the ethidium...Ch. 9 - The linear bacteriophage genomic DNA has at each...Ch. 9 - Consider a partial restriction digestion, in which...Ch. 9 - The text stated that molecular biologists have...
Ch. 9 - a. What is the purpose of molecular cloning? b....Ch. 9 - a. DNA polymerase b. RNA polymerase c. A...Ch. 9 - Is it possible that two different restriction...Ch. 9 - A plasmid vector pBS281 is cleaved by the enzyme...Ch. 9 - A recombinant DNA molecule is constructed using a...Ch. 9 - Suppose you are using a plasmid cloning vector...Ch. 9 - Prob. 17PCh. 9 - The lacZ gene from E. coli encodes the enzyme...Ch. 9 - Your undergraduate research advisor has assigned...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - You use the primer 5 GCCTCGAATCGGGTACC 3 to...Ch. 9 - a. To make a genomic library useful for sequencing...Ch. 9 - Problem 15 showed part of the sequence of the...Ch. 9 - Eukaryotic genomes are replete with repetitive...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Ch.21 What causes patients infected with the yellow fever virus to turn yellow (jaundice)? A. low blood pressure and anemia B. excess leukocytes C. alteration of skin pigments D. liver damage in final stage of disease — What is the advantage for malarial parasites to grow and replicate in red blood cells? A. able to spread quickly B. able to avoid immune detection C. low oxygen environment for growth D. cooler area of the body for growth — Which microbe does not live part of its lifecycle outside humans? A. Toxoplasma gondii B. Cytomegalovirus C. Francisella tularensis D. Plasmodium falciparum — explain your answer thoroughlyarrow_forwardCh.22 Streptococcus pneumoniae has a capsule to protect it from killing by alveolar macrophages, which kill bacteria by… A. cytokines B. antibodies C. complement D. phagocytosis — What fact about the influenza virus allows the dramatic antigenic shift that generates novel strains? A. very large size B. enveloped C. segmented genome D. over 100 genes — explain your answer thoroughlyarrow_forwardWhat is this?arrow_forward
- Molecular Biology A-C components of the question are corresponding to attached image labeled 1. D component of the question is corresponding to attached image labeled 2. For a eukaryotic mRNA, the sequences is as follows where AUGrepresents the start codon, the yellow is the Kozak sequence and (XXX) just represents any codonfor an amino acid (no stop codons here). G-cap and polyA tail are not shown A. How long is the peptide produced?B. What is the function (a sentence) of the UAA highlighted in blue?C. If the sequence highlighted in blue were changed from UAA to UAG, how would that affecttranslation? D. (1) The sequence highlighted in yellow above is moved to a new position indicated below. Howwould that affect translation? (2) How long would be the protein produced from this new mRNA? Thank youarrow_forwardMolecular Biology Question Explain why the cell doesn’t need 61 tRNAs (one for each codon). Please help. Thank youarrow_forwardMolecular Biology You discover a disease causing mutation (indicated by the arrow) that alters splicing of its mRNA. This mutation (a base substitution in the splicing sequence) eliminates a 3’ splice site resulting in the inclusion of the second intron (I2) in the final mRNA. We are going to pretend that this intron is short having only 15 nucleotides (most introns are much longer so this is just to make things simple) with the following sequence shown below in bold. The ( ) indicate the reading frames in the exons; the included intron 2 sequences are in bold. A. Would you expected this change to be harmful? ExplainB. If you were to do gene therapy to fix this problem, briefly explain what type of gene therapy youwould use to correct this. Please help. Thank youarrow_forward
- Molecular Biology Question Please help. Thank you Explain what is meant by the term “defective virus.” Explain how a defective virus is able to replicate.arrow_forwardMolecular Biology Explain why changing the codon GGG to GGA should not be harmful. Please help . Thank youarrow_forwardStage Percent Time in Hours Interphase .60 14.4 Prophase .20 4.8 Metaphase .10 2.4 Anaphase .06 1.44 Telophase .03 .72 Cytukinesis .01 .24 Can you summarize the results in the chart and explain which phases are faster and why the slower ones are slow?arrow_forward
- Can you circle a cell in the different stages of mitosis? 1.prophase 2.metaphase 3.anaphase 4.telophase 5.cytokinesisarrow_forwardWhich microbe does not live part of its lifecycle outside humans? A. Toxoplasma gondii B. Cytomegalovirus C. Francisella tularensis D. Plasmodium falciparum explain your answer thoroughly.arrow_forwardSelect all of the following that the ablation (knockout) or ectopoic expression (gain of function) of Hox can contribute to. Another set of wings in the fruit fly, duplication of fingernails, ectopic ears in mice, excess feathers in duck/quail chimeras, and homeosis of segment 2 to jaw in Hox2a mutantsarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Biology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage Learning
Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage Learning
BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
Concepts of BiologyBiologyISBN:9781938168116Author:Samantha Fowler, Rebecca Roush, James WisePublisher:OpenStax College
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Biology Today and Tomorrow without Physiology (Mi...
Biology
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Concepts of Biology
Biology
ISBN:9781938168116
Author:Samantha Fowler, Rebecca Roush, James Wise
Publisher:OpenStax College
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
DNA vs RNA (Updated); Author: Amoeba Sisters;https://www.youtube.com/watch?v=JQByjprj_mA;License: Standard youtube license