Nester's Microbiology: A Human Perspective
Nester's Microbiology: A Human Perspective
8th Edition
ISBN: 9780073522593
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 9, Problem 5MC
Summary Introduction

Introduction:

Vectors are the carrier of DNA (deoxyribonucleic acid) insert thatreplicates itself in an appropriate host cell. The gene insert is integrated into the vector that aims either to obtainthe product or the numerous copies of the DNA insert. LacZ´ gene promotes the selection by cleaving the disaccharide lactose molecule in an operon system. AslacZ´ gene is present in the plasmid of host cell encodes β-galactosidase cleaves X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside) and forms blue colonies when grown on culture plate with IPTG (isopropyl-β-D-1-thiogalactopyranoside) as an inducer.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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