Vectors are the carrier of DNA (deoxyribonucleic acid) insert thatreplicates itself in an appropriate host cell. The gene insert is integrated into the vector that aims either to obtainthe product or the numerous copies of the DNA insert. LacZ´ gene promotes the selection by cleaving the disaccharide lactose molecule in an operon system. AslacZ´ gene is present in the plasmid of host cell encodes β-galactosidase cleaves X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside) and forms blue colonies when grown on culture plate with IPTG (isopropyl-β-D-1-thiogalactopyranoside) as an inducer.

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Chapter 9, Problem 5MC

Summary Introduction

Introduction:

Vectors are the carrier of DNA (deoxyribonucleic acid) insert thatreplicates itself in an appropriate host cell. The gene insert is integrated into the vector that aims either to obtainthe product or the numerous copies of the DNA insert. LacZ´ gene promotes the selection by cleaving the disaccharide lactose molecule in an operon system. AslacZ´ gene is present in the plasmid of host cell encodes β-galactosidase cleaves X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside) and forms blue colonies when grown on culture plate with IPTG (isopropyl-β-D-1-thiogalactopyranoside) as an inducer.

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Answer 2

Question

Chapter 9, Problem 5MC

Summary Introduction

Introduction:

Vectors are the carrier of DNA (deoxyribonucleic acid) insert thatreplicates itself in an appropriate host cell. The gene insert is integrated into the vector that aims either to obtainthe product or the numerous copies of the DNA insert. LacZ´ gene promotes the selection by cleaving the disaccharide lactose molecule in an operon system. AslacZ´ gene is present in the plasmid of host cell encodes β-galactosidase cleaves X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside) and forms blue colonies when grown on culture plate with IPTG (isopropyl-β-D-1-thiogalactopyranoside) as an inducer.

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Answer 3

Question

Chapter 9, Problem 5MC

Summary Introduction

Introduction:

Vectors are the carrier of DNA (deoxyribonucleic acid) insert thatreplicates itself in an appropriate host cell. The gene insert is integrated into the vector that aims either to obtainthe product or the numerous copies of the DNA insert. LacZ´ gene promotes the selection by cleaving the disaccharide lactose molecule in an operon system. AslacZ´ gene is present in the plasmid of host cell encodes β-galactosidase cleaves X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside) and forms blue colonies when grown on culture plate with IPTG (isopropyl-β-D-1-thiogalactopyranoside) as an inducer.

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Answer 4

Question

Chapter 9, Problem 5MC

Summary Introduction

Introduction:

Vectors are the carrier of DNA (deoxyribonucleic acid) insert thatreplicates itself in an appropriate host cell. The gene insert is integrated into the vector that aims either to obtainthe product or the numerous copies of the DNA insert. LacZ´ gene promotes the selection by cleaving the disaccharide lactose molecule in an operon system. AslacZ´ gene is present in the plasmid of host cell encodes β-galactosidase cleaves X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside) and forms blue colonies when grown on culture plate with IPTG (isopropyl-β-D-1-thiogalactopyranoside) as an inducer.

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Answer 5

Chapter 9, Problem 5MC

Summary Introduction

Introduction:

Vectors are the carrier of DNA (deoxyribonucleic acid) insert thatreplicates itself in an appropriate host cell. The gene insert is integrated into the vector that aims either to obtainthe product or the numerous copies of the DNA insert. LacZ´ gene promotes the selection by cleaving the disaccharide lactose molecule in an operon system. AslacZ´ gene is present in the plasmid of host cell encodes β-galactosidase cleaves X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside) and forms blue colonies when grown on culture plate with IPTG (isopropyl-β-D-1-thiogalactopyranoside) as an inducer.

Answer 6

Chapter 9, Problem 5MC

Summary Introduction

Introduction:

Vectors are the carrier of DNA (deoxyribonucleic acid) insert thatreplicates itself in an appropriate host cell. The gene insert is integrated into the vector that aims either to obtainthe product or the numerous copies of the DNA insert. LacZ´ gene promotes the selection by cleaving the disaccharide lactose molecule in an operon system. AslacZ´ gene is present in the plasmid of host cell encodes β-galactosidase cleaves X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside) and forms blue colonies when grown on culture plate with IPTG (isopropyl-β-D-1-thiogalactopyranoside) as an inducer.

Answer 7

Chapter 9, Problem 5MC

Summary Introduction

Introduction:

Vectors are the carrier of DNA (deoxyribonucleic acid) insert thatreplicates itself in an appropriate host cell. The gene insert is integrated into the vector that aims either to obtainthe product or the numerous copies of the DNA insert. LacZ´ gene promotes the selection by cleaving the disaccharide lactose molecule in an operon system. AslacZ´ gene is present in the plasmid of host cell encodes β-galactosidase cleaves X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside) and forms blue colonies when grown on culture plate with IPTG (isopropyl-β-D-1-thiogalactopyranoside) as an inducer.

Answer 8

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Answer 11

Ch. 9 - 1. Why are restriction enzymes useful in...Ch. 9 - Describe three general uses of genetically...Ch. 9 - Describe the function of a reporter gene.Ch. 9 - Describe three uses of genetically engineered...Ch. 9 - Prob. 5SA Ch. 9 - 6. What is cDNA? Why is it used when cloning...Ch. 9 - How many different temperatures are used in each...Ch. 9 - 8. How does PCR eventually generate a...Ch. 9 - Prob. 9SA Ch. 9 - 10. How does a DNA microarray function as a set...

Solution Summary: The author explains that vectors are the carrier of DNA insert thatreplicates itself in an appropriate host cell. LacZ gene promotes the selection by cleaving the disaccharide lactose molecule

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