Campbell Essential Biology with Physiology (5th Edition)
Campbell Essential Biology with Physiology (5th Edition)
5th Edition
ISBN: 9780321967671
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece, Kelly A. Hogan
Publisher: PEARSON
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Chapter 9, Problem 4SQ
Summary Introduction

Introduction: The genes present on the chromosomes have alleles. They define the phenotypic and genotypic traits of the person. The types of alleles include dominant and recessive condition, which are depicted by a capital letter and a small letter for, example B for a dominant allele and b for a recessive allele of a gene. The combination of both the genes after recombination defines the phenotype or the external expression of the characters of a particular person. The dominant allele of a gene expresses to form a protein, whereas the recessive allele does not express and is not involved in the phenotypic expression of the characters of a person. The recessive allele is expressed only in the homozygous recessive state, when the genes for the dominant phenotype are not present.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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