Chapter 9, Problem 1DAA

Summary Introduction

To determine: The type of cells that had the greatest response to an increase in concentration of the engineered RIP.

Introduction: Ribosome-inactivating proteins (RIPs) inactivate the ribosomes and prevent protein synthesis in a cell. It removes adenine base from ribosomal rRNA that forms the part of the binding site for proteins involved in GTP. This results in inability of ribosome to bind to these proteins and protein synthesis stops. The toxic RIPs have a domain that makes them enter into another that interferes with ribosome. They have antiviral and anticancer properties, and are used to design drugs for HIV and cancer.