Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 9, Problem 1CA
Endospores appear as colorless areas in a simple stain and in a
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Chapter 9 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 9 - What are the Gram reactions of Clostridium and...Ch. 9 - How might a capsule contribute to pathogenicity?...Ch. 9 - Prob. 3QCh. 9 - The flagella stain uses crystal violet, as does...Ch. 9 - How would an endospore stain of Mycobacterium...Ch. 9 - What type of culture medium would increase the...Ch. 9 - Prob. 3CTCh. 9 - Prob. 4CTCh. 9 - Endospores appear as colorless areas in a simple...
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- What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.arrow_forwardIs it possible to perform double or triple staining using fluorescent dyes? Explain.arrow_forwardDescribe the bacteria observed in different staining procedures. Please refer to the photo - Endospore Stain - Capsule Stainarrow_forward
- What's the difference between the gram stain of Bacillus megaterium and Bacillus Subtilis?arrow_forwardWhy is it necessary to perform a negative stain to visualize capsules?arrow_forwardWhy is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet only?.arrow_forward
- Why are basic dyes more effective for bacterial staining than acidic dyes?arrow_forwardWhy can’t gram staining be used for mycobacterium?arrow_forwardWhich of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.arrow_forward
- For each of the following stains, list each of the chemicals used (in order), briefly describe what you would observe under the microscope, and list examples for each. - Gram stain (list color and example for Gram + and Gram -) - Acid Fast (list color and example for Acid Fast + and -) - Negative Stain (list color of organism stained with negative stain) - Capsule Stain (list color and example of organism with capsule) - Spore stain (list color and example of spore and cell)arrow_forwardDifferentiate the staining procedures. Gram Stain Acid Fast Endospore Capsule Flagella a) Principle b) Characteristic of Bacteria viewed using this stain. (e.g. bacterial type, strain, or targeting particular bacterial feature/s) c) Stain/s Used d) Type of Stain e) Mechanism of Staining (of each stain used) f) Flow of Methodarrow_forwardHow can colony morphology be used in the identification of bacterial species? What are the environmental factors that affect the growth of bacteria? What is agar (or agar-agar)? Is this different from nutrient agar? Why? How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage controlarrow_forward
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