Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired and suspect that a mutation has occurred in an enzyme found at the replication fork. Which enzyme is most likely to be mutated?

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Answer 1

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Chapter 9, Problem 1ACQ

Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired and suspect that a mutation has occurred in an enzyme found at the replication fork. Which enzyme is most likely to be mutated?

Chapter 9, Problem 1ACQ, Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired

Expert Solution & Answer

Summary Introduction

To write:

The enzyme which is most likely mutated when joining of Okazaki fragments is impaired in the replication.

Introduction:

DNA replication is the process in which the copy of DNA is made. This is done in 3 steps in a eukaryotic cell. They are initiation, elongation, and termination. It is a semiconservative type of replication. Enzymes involved in this process are helicase, DNA polymerase, and DNA ligase.

Explanation of Solution

DNA replication is started at the origin of replication where a Y shaped fork known as replication is formed when both strands are separated by helicase enzyme. Both the strands of DNA act as a template. One strand is known as a leading strand at which replication is continuous and other strand is known as a lagging strand at which replication occurs in fragments. This is because DNA polymerase can synthesize DNA only in 5' to 3' direction. These fragments are known as Okazaki fragments. These fragments at the lagging strands are then sealed by the enzyme DNA ligase. The joining of Okazaki fragments is impaired when the DNA ligase is not able to join the strands. This can occur due to the mutation in enzyme DNA ligase.

Conclusion

When joining of DNA fragments on the lagging strand is impaired in DNA replication, the mutation is most likely to occur in DNA ligase as it is an important enzyme which joins the strands.

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After you feel comfortable with your counting method and identifying cells in the various stages of mitosis, use the four images below of whitefish blastula to count the cells in each stage until you reach 100 total cells, recording your data below in Data Table 1. (You may not need to use all four images. Stop counting when you reach 100 total cells.) After totaling the cells in each stage, calculate the percent of cells in each stage. (Divide total of stage by overall total of 100 and then multiply by 100 to obtain percentage.) Data Table 1Stage Totals PercentInterphase Mitosis: Prophase Metaphase Anaphase Telophase Cytokinesis Totals 100 100% To find the length of time whitefish blastula cells spend in each stage, multiply the percent (recorded as a decimal, in other words take the percent number and divide by 100) by 24 hours. (Example: If percent is 20%, then Time in Hours = .2 * 24 = 4.8) Record your data in Data…

What are Clathrin coated vesicles and what is their function?

Answer 2

Textbook Question

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Chapter 9, Problem 1ACQ

Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired and suspect that a mutation has occurred in an enzyme found at the replication fork. Which enzyme is most likely to be mutated?

Chapter 9, Problem 1ACQ, Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired

Expert Solution & Answer

Summary Introduction

To write:

The enzyme which is most likely mutated when joining of Okazaki fragments is impaired in the replication.

Introduction:

DNA replication is the process in which the copy of DNA is made. This is done in 3 steps in a eukaryotic cell. They are initiation, elongation, and termination. It is a semiconservative type of replication. Enzymes involved in this process are helicase, DNA polymerase, and DNA ligase.

Explanation of Solution

DNA replication is started at the origin of replication where a Y shaped fork known as replication is formed when both strands are separated by helicase enzyme. Both the strands of DNA act as a template. One strand is known as a leading strand at which replication is continuous and other strand is known as a lagging strand at which replication occurs in fragments. This is because DNA polymerase can synthesize DNA only in 5' to 3' direction. These fragments are known as Okazaki fragments. These fragments at the lagging strands are then sealed by the enzyme DNA ligase. The joining of Okazaki fragments is impaired when the DNA ligase is not able to join the strands. This can occur due to the mutation in enzyme DNA ligase.

Conclusion

When joining of DNA fragments on the lagging strand is impaired in DNA replication, the mutation is most likely to occur in DNA ligase as it is an important enzyme which joins the strands.

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Answer 3

Textbook Question

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Chapter 9, Problem 1ACQ

Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired and suspect that a mutation has occurred in an enzyme found at the replication fork. Which enzyme is most likely to be mutated?

Chapter 9, Problem 1ACQ, Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired

Expert Solution & Answer

Summary Introduction

To write:

The enzyme which is most likely mutated when joining of Okazaki fragments is impaired in the replication.

Introduction:

DNA replication is the process in which the copy of DNA is made. This is done in 3 steps in a eukaryotic cell. They are initiation, elongation, and termination. It is a semiconservative type of replication. Enzymes involved in this process are helicase, DNA polymerase, and DNA ligase.

Explanation of Solution

DNA replication is started at the origin of replication where a Y shaped fork known as replication is formed when both strands are separated by helicase enzyme. Both the strands of DNA act as a template. One strand is known as a leading strand at which replication is continuous and other strand is known as a lagging strand at which replication occurs in fragments. This is because DNA polymerase can synthesize DNA only in 5' to 3' direction. These fragments are known as Okazaki fragments. These fragments at the lagging strands are then sealed by the enzyme DNA ligase. The joining of Okazaki fragments is impaired when the DNA ligase is not able to join the strands. This can occur due to the mutation in enzyme DNA ligase.

Conclusion

When joining of DNA fragments on the lagging strand is impaired in DNA replication, the mutation is most likely to occur in DNA ligase as it is an important enzyme which joins the strands.

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Answer 4

Textbook Question

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Chapter 9, Problem 1ACQ

Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired and suspect that a mutation has occurred in an enzyme found at the replication fork. Which enzyme is most likely to be mutated?

Chapter 9, Problem 1ACQ, Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired

Expert Solution & Answer

Summary Introduction

To write:

The enzyme which is most likely mutated when joining of Okazaki fragments is impaired in the replication.

Introduction:

DNA replication is the process in which the copy of DNA is made. This is done in 3 steps in a eukaryotic cell. They are initiation, elongation, and termination. It is a semiconservative type of replication. Enzymes involved in this process are helicase, DNA polymerase, and DNA ligase.

Explanation of Solution

DNA replication is started at the origin of replication where a Y shaped fork known as replication is formed when both strands are separated by helicase enzyme. Both the strands of DNA act as a template. One strand is known as a leading strand at which replication is continuous and other strand is known as a lagging strand at which replication occurs in fragments. This is because DNA polymerase can synthesize DNA only in 5' to 3' direction. These fragments are known as Okazaki fragments. These fragments at the lagging strands are then sealed by the enzyme DNA ligase. The joining of Okazaki fragments is impaired when the DNA ligase is not able to join the strands. This can occur due to the mutation in enzyme DNA ligase.

Conclusion

When joining of DNA fragments on the lagging strand is impaired in DNA replication, the mutation is most likely to occur in DNA ligase as it is an important enzyme which joins the strands.

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Answer 5

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Answer 6

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Answer 7

Expert Solution & Answer

Summary Introduction

To write:

The enzyme which is most likely mutated when joining of Okazaki fragments is impaired in the replication.

Introduction:

DNA replication is the process in which the copy of DNA is made. This is done in 3 steps in a eukaryotic cell. They are initiation, elongation, and termination. It is a semiconservative type of replication. Enzymes involved in this process are helicase, DNA polymerase, and DNA ligase.

Explanation of Solution

DNA replication is started at the origin of replication where a Y shaped fork known as replication is formed when both strands are separated by helicase enzyme. Both the strands of DNA act as a template. One strand is known as a leading strand at which replication is continuous and other strand is known as a lagging strand at which replication occurs in fragments. This is because DNA polymerase can synthesize DNA only in 5' to 3' direction. These fragments are known as Okazaki fragments. These fragments at the lagging strands are then sealed by the enzyme DNA ligase. The joining of Okazaki fragments is impaired when the DNA ligase is not able to join the strands. This can occur due to the mutation in enzyme DNA ligase.

Conclusion

When joining of DNA fragments on the lagging strand is impaired in DNA replication, the mutation is most likely to occur in DNA ligase as it is an important enzyme which joins the strands.

Answer 8

Expert Solution & Answer

Summary Introduction

To write:

The enzyme which is most likely mutated when joining of Okazaki fragments is impaired in the replication.

Introduction:

DNA replication is the process in which the copy of DNA is made. This is done in 3 steps in a eukaryotic cell. They are initiation, elongation, and termination. It is a semiconservative type of replication. Enzymes involved in this process are helicase, DNA polymerase, and DNA ligase.

Explanation of Solution

DNA replication is started at the origin of replication where a Y shaped fork known as replication is formed when both strands are separated by helicase enzyme. Both the strands of DNA act as a template. One strand is known as a leading strand at which replication is continuous and other strand is known as a lagging strand at which replication occurs in fragments. This is because DNA polymerase can synthesize DNA only in 5' to 3' direction. These fragments are known as Okazaki fragments. These fragments at the lagging strands are then sealed by the enzyme DNA ligase. The joining of Okazaki fragments is impaired when the DNA ligase is not able to join the strands. This can occur due to the mutation in enzyme DNA ligase.

Conclusion

When joining of DNA fragments on the lagging strand is impaired in DNA replication, the mutation is most likely to occur in DNA ligase as it is an important enzyme which joins the strands.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

To write:

The enzyme which is most likely mutated when joining of Okazaki fragments is impaired in the replication.

Introduction:

DNA replication is the process in which the copy of DNA is made. This is done in 3 steps in a eukaryotic cell. They are initiation, elongation, and termination. It is a semiconservative type of replication. Enzymes involved in this process are helicase, DNA polymerase, and DNA ligase.

Answer 12

Explanation of Solution

DNA replication is started at the origin of replication where a Y shaped fork known as replication is formed when both strands are separated by helicase enzyme. Both the strands of DNA act as a template. One strand is known as a leading strand at which replication is continuous and other strand is known as a lagging strand at which replication occurs in fragments. This is because DNA polymerase can synthesize DNA only in 5' to 3' direction. These fragments are known as Okazaki fragments. These fragments at the lagging strands are then sealed by the enzyme DNA ligase. The joining of Okazaki fragments is impaired when the DNA ligase is not able to join the strands. This can occur due to the mutation in enzyme DNA ligase.

Answer 13

Conclusion

When joining of DNA fragments on the lagging strand is impaired in DNA replication, the mutation is most likely to occur in DNA ligase as it is an important enzyme which joins the strands.

Answer 14

Ch. 9 - Figure 9.10 You isolate a cell strain in which the...Ch. 9 - Which of the following does cytosine pair with? a....Ch. 9 - Prokaryotes contain a ______ chromosome, and...Ch. 9 - DNA replicates by which of the following models?...Ch. 9 - The initial mechanism for repairing nucleotide...Ch. 9 - A promoter is ______. a. a specific sequence of...Ch. 9 - Portions of eukaryotic mRNA sequence that are...Ch. 9 - The RNA components of ribosomes are synthesized in...Ch. 9 - How long would the peptide be that is translated...Ch. 9 - Control of gene expression in eukaryotic cells...

Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired and suspect that a mutation has occurred in an enzyme found at the replication fork. Which enzyme is most likely to be mutated?

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