Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 9, Problem 10P

Answers to all problems are at the end of this book. Detailed solutions are available in the Student Solutions Manual, Study Guide, and Problems Book.

Determining 'the Concentration Limits of an Active Transport System

(Integrates with Chapter 3.) Fructose is present outside a Cell at 1 μM concentration. An active transport system in the plasma membrane transports fructose into this cell, using the free energy of ATP hydrolysis to drive fructose uptake. What is the highest intracellular concentration of fructose that this transported system can generate? Assume that one fructose is transported per ATP hydrolyzed; that ATP is hydrolyzed on the intracellular surface of the membrane; and that the concentrations of ATP, ADP, and Pi are 3mM, 1mM, and 0.5mM, respectively. T = 298 K. [Hint: Refer to Chapter 3 to recall the effects of concentration on free energy of ATP hydrolysis.)

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