PRINCIPLES OF LIFE-ACHIEVE ACCESS 2 TERM
PRINCIPLES OF LIFE-ACHIEVE ACCESS 2 TERM
3rd Edition
ISBN: 9781319450380
Author: HILLIS
Publisher: MAC HIGHER
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Chapter 8.4, Problem 1RA
Summary Introduction

To determine: The three different ways in which genes can be altered naturally in a bacterial cell.

Introduction: Conjugation is the process through which the transfer of DNA occurs directly from one cell to another. This can occur through cell-to-cell contact.

Summary Introduction

To determine: The mechanism that can occur in any bacterial cell.

Introduction: The transfer of genes from one individual to another without undergoing sexual reproduction is called lateral gene transfer. On the contrary, the vertical gene transfer involves the transfer of genes from parents to offsprings.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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