Chapter 8, Problem 7Q

Summary Introduction

To determine: How does the cleavage of a repressor at a site between DNA-binding domain and dimerization domain result in its dissociation from DNA.

Introduction:

Lysogeny also known as prophage stage refers to integration of phage in the host genome where it replicates with the host DNA over many generations. To maintain this state, the lambda (λ) repressor represses the virus’s lytic genes. Repressor molecules contain an N-terminal domain that binds to DNA and a C-terminal dimerization domain. They bind as dimers to critical sites on the lambda (λ) genome.