Biochemistry
Biochemistry
8th Edition
ISBN: 9781285429106
Author: Campbell, Mary K., FARRELL, Shawn O.
Publisher: Cengage Learning,
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Chapter 8, Problem 28RE
Interpretation Introduction

Interpretation:

Whether, or not, the relative amounts of cholesterol and phosphatidylcholine are the same in all membrane types present in a mammalian cell is to be checked.

Concept introduction:

Cholesterol is a steroid molecule that consists of four hydrocarbon rings instead of a linear hydrocarbon chain.

Phosphatidylcholine is a type of phospholipid that contains a polar head group, that is, choline, and a fatty acid chain.

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In 1956, E. P. Kennedy and S. B. Weiss published their study of membrane lipid phosphatidylcholine (lecithin) synthesis in rat liver. Their hypothesis was that phosphocholine joined with some cellular component to yield lecithin. In an earlier experiment, incubating 32 P-labeled phosphocholine at physiological temperature (37 °C) with broken cells from rat liver yielded labeled lecithin. This became their assay for the enzymes involved in lecithin synthesis. Determine the optimal pH for this enzyme and characterize the enzyme activity at different pH values. -O-P-O-CH2-CH₁₂-N(CH3)3 Phosphocholine H₂C-O-C-R HC-O-C-R2 + + + Cell fraction + ? HC-O-P-O-CH₁₂-CH₂-N(CH), O Phosphatidylcholine The researchers then centrifuged the broken cell preparation to separate the membranes from the soluble proteins. They tested three preparations: whole extract, membranes, and soluble proteins. Table 1 summarizes the results. Table 1: Cell fraction requirement for incorporation of 32p-phosphocholine into…
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Chapter 8 Solutions

Biochemistry

Ch. 8 - Prob. 11RECh. 8 - REFLECT AND APPLY Succulent plants from arid...Ch. 8 - REFLECT AND APPLY In the produce department of...Ch. 8 - REFLECT AND APPLY Egg yolks contain a high amount...Ch. 8 - REFLECT AND APPLY In the preparation of sauces...Ch. 8 - REFLECT AND APPLY When water birds have had their...Ch. 8 - RECALL Which of the following lipids are not found...Ch. 8 - RECALL Which of the following statements is (are)...Ch. 8 - REFLECT AND APPLY Why might some food companies...Ch. 8 - Prob. 20RECh. 8 - BIOCHEMICAL CONNECTIONS Crisco is made from...Ch. 8 - BIOCHEMICAL CONNECTIONS Why does the American...Ch. 8 - REFLECT AND APPLY In lipid bilayers, there is an...Ch. 8 - Prob. 24RECh. 8 - REFLECT AND APPLY Suggest a reason why the cell...Ch. 8 - REFLECT AND APPLY Suggest a reason why animals...Ch. 8 - REFLECT AND APPLY What is the energetic driving...Ch. 8 - Prob. 28RECh. 8 - RECALL How can fluorescence techniques be used to...Ch. 8 - Prob. 30RECh. 8 - Prob. 31RECh. 8 - REFLECT AND APPLY A membrane consists of 50%...Ch. 8 - REFLECT AND APPLY Suggest a reason why the same...Ch. 8 - REFLECTANDAPPLY Suppose that you are studying a...Ch. 8 - REFLECT AND APPLY Which statements are consistent...Ch. 8 - RECALL What role does phosphorylation of tyrosine...Ch. 8 - Prob. 37RECh. 8 - REFLECT AND APPLY Suggest a reason why inorganic...Ch. 8 - REFLECT AND APPLY Which statements are consistent...Ch. 8 - RECALL What is the structural relationship between...Ch. 8 - Prob. 41RECh. 8 - RECALL What are isoprene units? What do they have...Ch. 8 - Prob. 43RECh. 8 - Prob. 44RECh. 8 - Prob. 45RECh. 8 - Prob. 46RECh. 8 - Prob. 47RECh. 8 - Prob. 48RECh. 8 - REFLECT AND APPLY A health-conscious friend asks...Ch. 8 - Prob. 50RECh. 8 - Prob. 51RECh. 8 - Prob. 52RECh. 8 - REFLECT AND APPLY List two classes of compounds...Ch. 8 - BIOCHEMICAL CONNECTIONS Outline a possible...
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