Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 1CA
Clinical specimens suspected of containing Mycobacterium are digested with sodium hydroxide (NaOH) for 30 minutes prior to staining. Why is this technique used? Why isn’t this technique used for staining other bacteria?
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Chapter 8 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 8 - What are the large blue-stained areas on the...Ch. 8 - What is the decolorizing agent in the Gram stain?...Ch. 8 - Prob. 3QCh. 8 - What is phenol (carbolic acid), and what is its...Ch. 8 - Prob. 5QCh. 8 - How might the acid-fast characteristic of...Ch. 8 - In 1882, after experimenting with staining...Ch. 8 - Clinical specimens suspected of containing...Ch. 8 - The acid-fast stain is used to detect...
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- What staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.arrow_forwardWhy is oil immersion necessary to see bacteria in the laboratory?arrow_forwardDescribe the bacteria observed in different staining procedures. Please refer to the photo - Direct Stain - Negative Stainarrow_forward
- What staining method would you use to verify that the isolates are not Mycobacterium species? Describe the procedure as well. (arrow_forwardExplain why some cells are pink and others are purple in Gram-stained bacterial smear.arrow_forwardIn preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?arrow_forward
- Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?arrow_forwardWhat stain is used for mycobacterium?arrow_forwardAcid-Fast stain A) E. Coli -Morphology shape -Arragement -Acid-fast or non-Acid-fast B) S. Epidermidis -Morphology shape -Arragement -Acid-fast or non-Acid-fastarrow_forward
- What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic intestinal bacteria?arrow_forwardExplain the relationship between bacterial cell wall with gram-positive and gram-negative in the staining process. Please state chemical used and their function.arrow_forwardDESCRIBE THE BACTERIA OBSERVED. DO NOT DEFINE THE STAINING TECHNIQUES. PLEASE REFER TO THE PHOTO.arrow_forward
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