EBK HUMAN ANATOMY & PHYSIOLOGY
1st Edition
ISBN: 9780100659834
Author: AMERMAN
Publisher: YUZU
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Textbook Question
Chapter 7.2, Problem 6QC
Where are the six main fontanels located?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 7 Solutions
EBK HUMAN ANATOMY & PHYSIOLOGY
Ch. 7.1 - 1. Which parts of the skeleton belong to the...Ch. 7.1 - Where are skeletal cartilages located?Ch. 7.1 - 3. What are some functions of bone markings?
Ch. 7.2 - 1. Match each bone with the correct description...Ch. 7.2 - 2. Which bones form the orbit?
Ch. 7.2 - 3. What are the paranasal sinuses, and how are...Ch. 7.2 - 4. How are the oral and nasal cavities related...Ch. 7.2 - What are fontanels, and why are they important in...Ch. 7.2 - Where are the six main fontanels located?Ch. 7.2 - What is unique about the hyoid bone?
Ch. 7.3 - 1. How many cervical, thoracic, lumbar, sacral,...Ch. 7.3 - Prob. 2QCCh. 7.3 - Compare scoliosis, lordosis, and kyphosis.Ch. 7.3 - How do the atlas and axis differ from other...Ch. 7.3 - Identify each of the following characteristics as...Ch. 7.3 - 6. Describe the structure of an intervertebral...Ch. 7.3 - 7. What are the three components of the sternum?
Ch. 7.3 - How do true, false, and floating ribs differ?Ch. 7.4 - With which structures does the clavicle...Ch. 7.4 - 2. What are the glenoid cavity, acromion, and...Ch. 7.4 - 3. With which structures does the humerus...Ch. 7.4 - Describe the structure and location of the...Ch. 7.4 - 5. How do the radius and ulna differ in their...Ch. 7.4 - Which parts of the radius and ulna articulate with...Ch. 7.4 - With what other bones do the radius and ulna...Ch. 7.4 - 8. List the proximal and distal carpal bones from...Ch. 7.4 - 9. How many metacarpals and phalanges are in the...Ch. 7.4 - 10. What are the three parts of a metacarpal and...Ch. 7.5 - With which bones does the femur articulate? Be...Ch. 7.5 - Which parts of the femur form these articulations?Ch. 7.5 - Prob. 3QCCh. 7.5 - 4. With which bones does the tibia articulate?...Ch. 7.5 - 5. What are the bony projections of the medial...Ch. 7.5 - Prob. 6QCCh. 7.5 - How does the structure of the foot and toes...Ch. 7.5 - 8. What are the three arches of the foot?
Ch. 7 - 1. Which of the following are considered parts of...Ch. 7 - 2. ________is the anatomical name for a hole in a...Ch. 7 - Fill in the blanks: The two parietal bones are...Ch. 7 - Mark the following statements as true or false. If...Ch. 7 - The only moveable bone in the adult skull is the:...Ch. 7 - 6. The structure(s) that divide the nasal cavity...Ch. 7 - The soft spots in an infants skull are known as:...Ch. 7 - 8. Mark the following statements as true or...Ch. 7 - 9. Transverse foramina are a characteristic of...Ch. 7 - Fill in the blanks: The inferior portion of the...Ch. 7 - How do true, false, and floating ribs differ from...Ch. 7 - Which of the following portions of the scapula...Ch. 7 - Fill in the blanks: The only bone of the arm is...Ch. 7 - The elbow bone is called the: a. trochlea. b....Ch. 7 - Which of the following is not a proximal carpal...Ch. 7 - Mark the following statements as true or false. If...Ch. 7 - 17. The most lateral projection of the proximal...Ch. 7 - 18. Fill in the blanks: The bones of the leg are...Ch. 7 - 19. The heel bone is more properly known as...Ch. 7 - The arch(es) of the foot are the: a. transverse...Ch. 7 - How do the atlas (C1) and the axis (C2) differ...Ch. 7 - Explain how abnormal bone structure could affect...Ch. 7 - What structures form the knee and elbow joints? Of...Ch. 7 - A deviated septum results when the nasal septum is...Ch. 7 - Mrs. Dent presents to the clinic with back pain....Ch. 7 - You arrive on the scene where a person without a...Ch. 7 - Predict where each of the following structures is...
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