BIO 1408/09 PKG W/LS CODE
5th Edition
ISBN: 9781260370829
Author: Mader
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 7.1, Problem 1CYP
Explain why
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 7 Solutions
BIO 1408/09 PKG W/LS CODE
Ch. 7.1 - Explain the role of cellular respiration in a cellCh. 7.1 - State the overall reaction for glucose breakdown.Ch. 7.1 - List the four phases of cellular respiration, and...Ch. 7.1 - Explain why cellular respiration is needed by the...Ch. 7.1 - Prob. 2CYPCh. 7.1 - Summarize the four stages of cellular respiration,...Ch. 7.1 - Prob. 1ACh. 7.1 - Prob. 2ACh. 7.2 - Prob. 1LOCh. 7.2 - Prob. 2LO
Ch. 7.2 - Prob. 3LOCh. 7.2 - Prob. 1CYPCh. 7.2 - Contrast the energy-investment step of glycolysis...Ch. 7.2 - Prob. 3CYPCh. 7.2 - Prob. 3ACh. 7.2 - Prob. 4ACh. 7.3 - Explain why fermentation pathways are beneficial...Ch. 7.3 - Prob. 2LOCh. 7.3 - Prob. 1CYPCh. 7.3 - Prob. 2CYPCh. 7.3 - Prob. 3CYPCh. 7.3 - Prob. 5ACh. 7.3 - Prob. 6ACh. 7.4 - Prob. 1LOCh. 7.4 - Prob. 2LOCh. 7.4 - 3. Identify the inputs and outputs of each pathway...Ch. 7.4 - Prob. 4LOCh. 7.4 - Prob. 1CYPCh. 7.4 - Prob. 2CYPCh. 7.4 - Prob. 3CYPCh. 7.4 - Prob. 7ACh. 7.4 - Prob. 8ACh. 7.4 - Prob. 9ACh. 7.4 - Prob. 10ACh. 7.4 - Prob. 11ACh. 7.4 - Prob. 12ACh. 7.5 - Prob. 1LOCh. 7.5 - Prob. 2LOCh. 7.5 - Prob. 1CYPCh. 7.5 - Prob. 2CYPCh. 7.5 - Prob. 3CYPCh. 7.5 - Prob. 13ACh. 7.5 - Prob. 14ACh. 7.5 - Prob. 15ACh. 7 - Prob. 1BYBCh. 7 - Prob. 2BYBCh. 7 - How are other organic nutrients, such as fats and...Ch. 7 - Prob. S3.2BYBCh. 7 - Prob. S4.4BYBCh. 7 - Prob. S5.2BYBCh. 7 - The compound malonate , a substrate of the citric...Ch. 7 - Prob. 2TCCh. 7 - Prob. 3TCCh. 7 - Prob. 4TC
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