Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 7, Problem 8R
Summary Introduction
Introduction:
Disinfectant is a chemical agent which destroys bacteria and hinder its growth. Effective disinfectant should stop bacterial growth even in high dilution levels. When it reduces its effects and allows growth of bacteria, its usage become futile.
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In sterilization, which among the supplies, instruments, glassware, etc. under
the list of materials can be sterilized using either or both equipment below?
List them down.
For "autoclaving"
For Dry heat oven
Can be sterilized
only
sterilization
with either
Materials:
200-ml Erlenmeyer flask
Stove
500-ml Erlenmeyer flask
Autoclave
10-ml graduated cylinder
Analytical balance
100-ml graduated cylinder
pH meter
Spatula
Stirring rod
100-ml beaker
Test tubes
Distilled water
Petri dish
Stirring rod
Alcohol lamp
Glass dropper
A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-2 plate? Include units and use OCD formula.
What is the minimal concentration (MIC) for disinfectant number 1? Just write the corresponding number including the decimal as written.
ug/mL.
Disinfectant
1
2
3
4
concentration
0.1 ug/mL
+++
++++
++
+++
0.2 ug/mL
++
++
+
++
0.5 ug/mL
+
+
1.0 ug/mL
5.0 ug/mL
subculture
1
2
3
4
0.1 ug/mL
++++
++++
++++
++++
0.2 ug/mL
++++
++++
++++
++++
0.5 ug/mL
++++
++++
++++
1.0 ug/mL
++++
5.0 ug/mL
Submit response
Chapter 7 Solutions
Microbiology: An Introduction
Ch. 7 - The thermal death time for a suspension of...Ch. 7 - If pasteurization does not achieve sterilization,...Ch. 7 - Thermal death point is not considered an accurate...Ch. 7 - Prob. 4RCh. 7 - DRAW IT A bacterial culture was in log phase in...Ch. 7 - How do autoclaving, hot air, and pasteurization...Ch. 7 - How do salts and sugars preserve foods? Why are...Ch. 7 - Prob. 8RCh. 7 - A large hospital washes burn patients in a...Ch. 7 - Prob. 10R
Ch. 7 - Which of the following does not kill endospores?...Ch. 7 - Which of the following is most effective for...Ch. 7 - Prob. 3MCQCh. 7 - Which of the following cannot be used to sterilize...Ch. 7 - Prob. 5MCQCh. 7 - The data were obtained from a use-dilution test...Ch. 7 - The data were obtained from a use-dilution test...Ch. 7 - Prob. 8MCQCh. 7 - A classmate is trying to determine how a...Ch. 7 - Which of the following is most likely to be...Ch. 7 - Prob. 1ACh. 7 - For each of the following bacteria, explain why it...Ch. 7 - A use-dilution test was used to evaluate two...Ch. 7 - To determine the lethal action of microwave...Ch. 7 - Entamoeba histolytica and Giardia lamblia were...Ch. 7 - Between March 9 and April 12five chronic...Ch. 7 - Prob. 3CAE
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