
Concept explainers
In a genetics lab, Kim and Maria infected a sample from an E. coli culture with a particular virulent bacteriophage. They noticed that most of the cells were lysed, but a few survived. The survival rate in their sample was about 1 × 10-4. Kim was sure the bacteriophage induced the resistance in the cells, while Maria thought that resistant mutants probably already existed in the sample of cells they used. Earlier, for a different
experiment, they had spread a dilute suspension of E. coli onto solid medium in a large petri dish, and, after seeing that about 105 colonies were growing up, they had replica-plated that plate onto three other plates. Kim and Maria decide to use these plates to test their theories. They pipette a suspension of the bacteriophage onto each of the three replica plates. What should they see if Kim is right? What should they see if Maria is right?

Want to see the full answer?
Check out a sample textbook solution
Chapter 7 Solutions
EBK GENETICS: FROM GENES TO GENOMES
- Pleiotropic genes are genes that (blank) Cause a swapping of organs/structures, are the result of duplicated sets of chromosomes, never produce protein products, and have more than one purpose/functionarrow_forwardA loss of function mutation in Pitx1 enhancers can cause (blank) Removal of Pitx1 exons and growth of ectopic hindlimbs, growth of extra ectopic forelimbs, loss of forelimb specification and development, and loss of hindlimb specification and developmentarrow_forwardHox1a most likely contributes to (blank) patterning in the developing embryo? Ventral, posterior, limb or anteriorarrow_forward
- Select all of the following that can help establish Hox gene expression boundaries (things that affect Hox and not things that Hox affects). Retinoic acid, anterior/posterior axis, fibroblast growth factors, vagal neural crest, and enhancersarrow_forwardEctopic expression of Hox often results in (blank) phenotypes. (Blank) transformations are characterized by the replacement of one body part/structure with another. Hoxeotic, homealoneotic, joexotic, or homeoticarrow_forwardWhat's the difference when drawing omega-6 and omega-3?arrow_forward
- . Consider a base substitution mutation that occurred in a DNA sequence that resulted in a change in the encoded protein from the amino acid glutamic acid to aspartic acid. Normally the glutamic acid amino acid is located on the outside of the soluble protein but not near an active site. O-H¨ A. What type of mutation occurred? O-H B. What 2 types of chemical bonds are found in the R-groups of each amino acid? The R groups are shaded. CH2 CH2 CH2 H2N-C-COOH H2N-C-COOH 1 H Glutamic acid H Aspartic acid C. What 2 types of bonds could each R-group of each of these amino acids form with other molecules? D. Consider the chemical properties of the two amino acids and the location of the amino acid in the protein. Explain what effect this mutation will have on this protein's function and why.arrow_forwardengineered constructs that consist of hollow fibers are acting as synthetic capillaries, around which cells have been loaded. The cellular space around a single fiber can be modeled as if it were a Krogh tissue cylinder. Each fiber has an outside “capillary” radius of 100 µm and the “tissue” radius can be taken as 200 µm. The following values apply to the device:R0 = 20 µM/secaO2 = 1.35 µM/mmHgDO2,T = 1.67 x 10-5 cm2/secPO2,m = 4 x 10-3 cm/secInstead of blood inside the fibers, the oxygen transport and tissue consumption are being investigated by usingan aqueous solution saturated with pure oxygen. As a result, there is no mass transfer resistance in the synthetic“capillary”, only that due to the membrane itself. Rather than accounting for pO2 variations along the length ofthe fiber, use an average value in the “capillary” of 130 mmHg.Is the tissue fully oxygenated?arrow_forwardMolecular Biology Please help with question. thank you You are studying the expression of the lac operon. You have isolated mutants as described below. In the presence of glucose, explain/describe what would happen, for each mutant, to the expression of the lac operon when you add lactose AND what would happen when the bacteria has used up all of the lactose (if the mutant is able to use lactose).5. Mutations in the lac operator that strengthen the binding of the lac repressor 200 fold 6. Mutations in the promoter that prevent binding of RNA polymerase 7. Mutations in CRP/CAP protein that prevent binding of cAMP8. Mutations in sigma factor that prevent binding of sigma to core RNA polymerasearrow_forward
- Molecular Biology Please help and there is an attached image. Thank you. A bacteria has a gene whose protein/enzyme product is involved with the synthesis of a lipid necessary for the synthesis of the cell membrane. Expression of this gene requires the binding of a protein (called ACT) to a control sequence (called INC) next to the promoter. A. Is the expression/regulation of this gene an example of induction or repression?Please explain:B. Is this expression/regulation an example of positive or negative control?C. When the lipid is supplied in the media, the expression of the enzyme is turned off.Describe one likely mechanism for how this “turn off” is accomplished.arrow_forwardMolecular Biology Please help. Thank you. Discuss/define the following:(a) poly A polymerase (b) trans-splicing (c) operonarrow_forwardMolecular Biology Please help with question. Thank you in advance. Discuss, compare and contrast the structure of promoters inprokaryotes and eukaryotes.arrow_forward
- Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage LearningHuman Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage LearningBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
- Biology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage LearningBiology: The Unity and Diversity of Life (MindTap...BiologyISBN:9781305073951Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa StarrPublisher:Cengage Learning




