Concept explainers
To review:
What CRISPR is and how it is used to edit genes.
Introduction:
CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Explanation of Solution
Bacteria regularly incorporate bits of viral DNA into their own circular DNA. This way, when a viral particle infects them and injects its DNA, the bacteria produce RNA strands that are complementary to the viral DNA, which will bind to the viral DNA. The bacteria then use the enzyme Cas9 to cut the DNA bound by the RNA strands into fragments.
Taking inspiration from this immune mechanism of bacteria -
- The CRISPR technology makes use of a synthetic RNA guide molecule which can bind to the target DNA in a cell.
- The Cas9 enzyme (guided by the RNA molecule) is used to cut the DNA that has been bound by the RNA.
- A new gene can be inserted into this position to alter the cell in as needed.
- A plasmid carrying the DNA for the synthesis of the RNA guide and the Cas9 enzyme can be inserted into target cell for the whole process to occur.
This technology results in more precise editing of the gene.
CRISPR stands for “clustered regularly interspersed short palindromic repeats”, which refers to a type of DNA organization in viruses. This technique takes inspiration from this immune mechanism of bacteria, where the bacterial cell uses an RNA molecule to guide enzymes to cut invading DNA molecules.
Want to see more full solutions like this?
- ✓ Details Draw a protein that is embedded in a membrane (a transmembrane protein), label the lipid bilayer and the protein. Identify the areas of the lipid bilayer that are hydrophobic and hydrophilic. Draw a membrane with two transporters: a proton pump transporter that uses ATP to generate a proton gradient, and a second transporter that moves glucose by secondary active transport (cartoon-like is ok). It will be important to show protons moving in the correct direction, and that the transporter that is powered by secondary active transport is logically related to the proton pump.arrow_forwarddrawing chemical structure of ATP. please draw in and label whats asked. Thank you.arrow_forwardOutline the negative feedback loop that allows us to maintain a healthy water concentration in our blood. You may use diagram if you wisharrow_forward
- Give examples of fat soluble and non-fat soluble hormonesarrow_forwardJust click view full document and register so you can see the whole document. how do i access this. following from the previous question; https://www.bartleby.com/questions-and-answers/hi-hi-with-this-unit-assessment-psy4406-tp4-report-assessment-material-case-stydu-ms-alecia-moore.-o/5e09906a-5101-4297-a8f7-49449b0bb5a7. on Google this image comes up and i have signed/ payed for the service and unable to access the full document. are you able to copy and past to this response. please see the screenshot from google page. unfortunality its not allowing me attch the image can you please show me the mathmetic calculation/ workout for the reult sectionarrow_forwardIn tabular form, differentiate between reversible and irreversible cell injury.arrow_forward
- 1.)What cross will result in half homozygous dominant offspring and half heterozygous offspring? 2.) What cross will result in all heterozygous offspring?arrow_forward1.Steroids like testosterone and estrogen are nonpolar and large (~18 carbons). Steroids diffuse through membranes without transporters. Compare and contrast the remaining substances and circle the three substances that can diffuse through a membrane the fastest, without a transporter. Put a square around the other substance that can also diffuse through a membrane (1000x slower but also without a transporter). Molecule Steroid H+ CO₂ Glucose (C6H12O6) H₂O Na+ N₂ Size (Small/Big) Big Nonpolar/Polar/ Nonpolar lonizedarrow_forwardwhat are the answer from the bookarrow_forward
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage LearningConcepts of BiologyBiologyISBN:9781938168116Author:Samantha Fowler, Rebecca Roush, James WisePublisher:OpenStax College
- Biology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage LearningCase Studies In Health Information ManagementBiologyISBN:9781337676908Author:SCHNERINGPublisher:CengageBiology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage Learning



