What CRISPR is and how it is used to edit genes. Introduction: CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

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Answer 1

Question

Chapter 7, Problem 1SA

Summary Introduction

To review:

What CRISPR is and how it is used to edit genes.

Introduction:

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Expert Solution & Answer

Explanation of Solution

Bacteria regularly incorporate bits of viral DNA into their own circular DNA. This way, when a viral particle infects them and injects its DNA, the bacteria produce RNA strands that are complementary to the viral DNA, which will bind to the viral DNA. The bacteria then use the enzyme Cas9 to cut the DNA bound by the RNA strands into fragments.

Taking inspiration from this immune mechanism of bacteria -

The CRISPR technology makes use of a synthetic RNA guide molecule which can bind to the target DNA in a cell.
The Cas9 enzyme (guided by the RNA molecule) is used to cut the DNA that has been bound by the RNA.
A new gene can be inserted into this position to alter the cell in as needed.
A plasmid carrying the DNA for the synthesis of the RNA guide and the Cas9 enzyme can be inserted into target cell for the whole process to occur.

This technology results in more precise editing of the gene.

Conclusion

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, which refers to a type of DNA organization in viruses. This technique takes inspiration from this immune mechanism of bacteria, where the bacterial cell uses an RNA molecule to guide enzymes to cut invading DNA molecules.

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Students have asked these similar questions

The Snapdragon is a popular garden flower that comes in a variety of colours, including red, yellow, and orange. The genotypes and associated phenotypes for some of these flowers are as follows: aabb: yellow AABB, AABb, AaBb, and AaBB: red AAbb and Aabb: orange aaBB: yellow aaBb: ? Based on this information, what would the phenotype of a Snapdragon with the genotype aaBb be and why? Question 21 options: orange because A is epistatic to B yellow because A is epistatic to B red because B is epistatic to A orange because B is epistatic to A red because A is epistatic to B yellow because B is epistatic to A

A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation? The cell membranes are more resistant than normal. An isotonic solution had been added instead of water. A solution of 0.1 M NaCl had been added instead of water. Not enough water had been added to the red blood cell pellet. The man had sickle-cell anaemia.

Answer 2

Question

Chapter 7, Problem 1SA

Summary Introduction

To review:

What CRISPR is and how it is used to edit genes.

Introduction:

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Expert Solution & Answer

Explanation of Solution

Bacteria regularly incorporate bits of viral DNA into their own circular DNA. This way, when a viral particle infects them and injects its DNA, the bacteria produce RNA strands that are complementary to the viral DNA, which will bind to the viral DNA. The bacteria then use the enzyme Cas9 to cut the DNA bound by the RNA strands into fragments.

Taking inspiration from this immune mechanism of bacteria -

The CRISPR technology makes use of a synthetic RNA guide molecule which can bind to the target DNA in a cell.
The Cas9 enzyme (guided by the RNA molecule) is used to cut the DNA that has been bound by the RNA.
A new gene can be inserted into this position to alter the cell in as needed.
A plasmid carrying the DNA for the synthesis of the RNA guide and the Cas9 enzyme can be inserted into target cell for the whole process to occur.

This technology results in more precise editing of the gene.

Conclusion

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, which refers to a type of DNA organization in viruses. This technique takes inspiration from this immune mechanism of bacteria, where the bacterial cell uses an RNA molecule to guide enzymes to cut invading DNA molecules.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Question

Chapter 7, Problem 1SA

Summary Introduction

To review:

What CRISPR is and how it is used to edit genes.

Introduction:

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Expert Solution & Answer

Explanation of Solution

Bacteria regularly incorporate bits of viral DNA into their own circular DNA. This way, when a viral particle infects them and injects its DNA, the bacteria produce RNA strands that are complementary to the viral DNA, which will bind to the viral DNA. The bacteria then use the enzyme Cas9 to cut the DNA bound by the RNA strands into fragments.

Taking inspiration from this immune mechanism of bacteria -

The CRISPR technology makes use of a synthetic RNA guide molecule which can bind to the target DNA in a cell.
The Cas9 enzyme (guided by the RNA molecule) is used to cut the DNA that has been bound by the RNA.
A new gene can be inserted into this position to alter the cell in as needed.
A plasmid carrying the DNA for the synthesis of the RNA guide and the Cas9 enzyme can be inserted into target cell for the whole process to occur.

This technology results in more precise editing of the gene.

Conclusion

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, which refers to a type of DNA organization in viruses. This technique takes inspiration from this immune mechanism of bacteria, where the bacterial cell uses an RNA molecule to guide enzymes to cut invading DNA molecules.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Question

Chapter 7, Problem 1SA

Summary Introduction

To review:

What CRISPR is and how it is used to edit genes.

Introduction:

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Expert Solution & Answer

Explanation of Solution

Bacteria regularly incorporate bits of viral DNA into their own circular DNA. This way, when a viral particle infects them and injects its DNA, the bacteria produce RNA strands that are complementary to the viral DNA, which will bind to the viral DNA. The bacteria then use the enzyme Cas9 to cut the DNA bound by the RNA strands into fragments.

Taking inspiration from this immune mechanism of bacteria -

The CRISPR technology makes use of a synthetic RNA guide molecule which can bind to the target DNA in a cell.
The Cas9 enzyme (guided by the RNA molecule) is used to cut the DNA that has been bound by the RNA.
A new gene can be inserted into this position to alter the cell in as needed.
A plasmid carrying the DNA for the synthesis of the RNA guide and the Cas9 enzyme can be inserted into target cell for the whole process to occur.

This technology results in more precise editing of the gene.

Conclusion

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, which refers to a type of DNA organization in viruses. This technique takes inspiration from this immune mechanism of bacteria, where the bacterial cell uses an RNA molecule to guide enzymes to cut invading DNA molecules.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 7, Problem 1SA

Summary Introduction

To review:

What CRISPR is and how it is used to edit genes.

Introduction:

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Expert Solution & Answer

Explanation of Solution

Bacteria regularly incorporate bits of viral DNA into their own circular DNA. This way, when a viral particle infects them and injects its DNA, the bacteria produce RNA strands that are complementary to the viral DNA, which will bind to the viral DNA. The bacteria then use the enzyme Cas9 to cut the DNA bound by the RNA strands into fragments.

Taking inspiration from this immune mechanism of bacteria -

The CRISPR technology makes use of a synthetic RNA guide molecule which can bind to the target DNA in a cell.
The Cas9 enzyme (guided by the RNA molecule) is used to cut the DNA that has been bound by the RNA.
A new gene can be inserted into this position to alter the cell in as needed.
A plasmid carrying the DNA for the synthesis of the RNA guide and the Cas9 enzyme can be inserted into target cell for the whole process to occur.

This technology results in more precise editing of the gene.

Conclusion

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, which refers to a type of DNA organization in viruses. This technique takes inspiration from this immune mechanism of bacteria, where the bacterial cell uses an RNA molecule to guide enzymes to cut invading DNA molecules.

Answer 6

Chapter 7, Problem 1SA

Summary Introduction

To review:

What CRISPR is and how it is used to edit genes.

Introduction:

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Expert Solution & Answer

Explanation of Solution

Bacteria regularly incorporate bits of viral DNA into their own circular DNA. This way, when a viral particle infects them and injects its DNA, the bacteria produce RNA strands that are complementary to the viral DNA, which will bind to the viral DNA. The bacteria then use the enzyme Cas9 to cut the DNA bound by the RNA strands into fragments.

Taking inspiration from this immune mechanism of bacteria -

The CRISPR technology makes use of a synthetic RNA guide molecule which can bind to the target DNA in a cell.
The Cas9 enzyme (guided by the RNA molecule) is used to cut the DNA that has been bound by the RNA.
A new gene can be inserted into this position to alter the cell in as needed.
A plasmid carrying the DNA for the synthesis of the RNA guide and the Cas9 enzyme can be inserted into target cell for the whole process to occur.

This technology results in more precise editing of the gene.

Conclusion

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, which refers to a type of DNA organization in viruses. This technique takes inspiration from this immune mechanism of bacteria, where the bacterial cell uses an RNA molecule to guide enzymes to cut invading DNA molecules.

Answer 7

Chapter 7, Problem 1SA

Summary Introduction

To review:

What CRISPR is and how it is used to edit genes.

Introduction:

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Answer 8

Expert Solution & Answer

Explanation of Solution

Bacteria regularly incorporate bits of viral DNA into their own circular DNA. This way, when a viral particle infects them and injects its DNA, the bacteria produce RNA strands that are complementary to the viral DNA, which will bind to the viral DNA. The bacteria then use the enzyme Cas9 to cut the DNA bound by the RNA strands into fragments.

Taking inspiration from this immune mechanism of bacteria -

The CRISPR technology makes use of a synthetic RNA guide molecule which can bind to the target DNA in a cell.
The Cas9 enzyme (guided by the RNA molecule) is used to cut the DNA that has been bound by the RNA.
A new gene can be inserted into this position to alter the cell in as needed.
A plasmid carrying the DNA for the synthesis of the RNA guide and the Cas9 enzyme can be inserted into target cell for the whole process to occur.

This technology results in more precise editing of the gene.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Conclusion

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, which refers to a type of DNA organization in viruses. This technique takes inspiration from this immune mechanism of bacteria, where the bacterial cell uses an RNA molecule to guide enzymes to cut invading DNA molecules.

Answer 12

Ch. 7 - Prob. 1SA Ch. 7 - Prob. 2SA Ch. 7 - Prob. 3SA Ch. 7 - Prob. 4SA Ch. 7 - Prob. 5SA Ch. 7 - Prob. 6SA Ch. 7 - Prob. 7SA Ch. 7 - Prob. 8SA Ch. 7 - Prob. 1MC Ch. 7 - Prob. 2MC

What CRISPR is and how it is used to edit genes. Introduction: CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Concept explainers

Videos

To review:

What CRISPR is and how it is used to edit genes.

Introduction:

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Explanation of Solution

Want to see more full solutions like this?

Chapter 7 Solutions

What CRISPR is and how it is used to edit genes. Introduction: CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Concept explainers

Videos

To review: What CRISPR is and how it is used to edit genes. Introduction: CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Explanation of Solution

Want to see more full solutions like this?

Chapter 7 Solutions

To review:

What CRISPR is and how it is used to edit genes.

Introduction:

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.