What Is Life? A Guide to Biology with Physiology
What Is Life? A Guide to Biology with Physiology
4th Edition
ISBN: 9781319065447
Author: Jay Phelan
Publisher: W. H. Freeman
bartleby

Concept explainers

bartleby

Videos

Question
Book Icon
Chapter 7, Problem 1SA
Summary Introduction

To review:

What CRISPR is and how it is used to edit genes.

Introduction:

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, referring to the way DNA was originally organized in some viruses.

Expert Solution & Answer
Check Mark

Explanation of Solution

Bacteria regularly incorporate bits of viral DNA into their own circular DNA. This way, when a viral particle infects them and injects its DNA, the bacteria produce RNA strands that are complementary to the viral DNA, which will bind to the viral DNA. The bacteria then use the enzyme Cas9 to cut the DNA bound by the RNA strands into fragments.

Taking inspiration from this immune mechanism of bacteria -

  1. The CRISPR technology makes use of a synthetic RNA guide molecule which can bind to the target DNA in a cell.
  2. The Cas9 enzyme (guided by the RNA molecule) is used to cut the DNA that has been bound by the RNA.
  3. A new gene can be inserted into this position to alter the cell in as needed.
  4. A plasmid carrying the DNA for the synthesis of the RNA guide and the Cas9 enzyme can be inserted into target cell for the whole process to occur.

This technology results in more precise editing of the gene.

Conclusion

CRISPR stands for “clustered regularly interspersed short palindromic repeats”, which refers to a type of DNA organization in viruses. This technique takes inspiration from this immune mechanism of bacteria, where the bacterial cell uses an RNA molecule to guide enzymes to cut invading DNA molecules.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!
Students have asked these similar questions
Which of the structural components listed in the Essential terms of section 1.3 (Cell components) could occur in a plant cell? Paragraph く BIUA 川く く 80 + кл K
Which of the following statements refer(s) directly to the cell theory? (Note that one or more correct answers are possible.) Select 2 correct answer(s) a) There are major differences between plant and animal cells. b) There are major differences between prokaryote and eukaryote cells. c) All cells have a cell wall. d) All cells have a cell membrane. e) Animals are composed of cells. f) When a bacterial cell divides, it produces two daughter cells.
Preoperative Diagnosis: Torn medial meniscus, left knee Postoperative Diagnosis: Combination horizontal cleavage tear/flap tear, posterior horn, medial meniscus, left knee. Operation: Arthroscopic subtotal medial meniscectomy, left knee Anesthetic: General endotracheal Description of Procedure: The patient was placed on the operating table in the supine position and general endotracheal anesthesia was administered. After an adequate level of anesthesia was achieved, the patient's left lower extremity was prepped with Betadine scrubbing solution, then draped in a sterile manner. Several sites were then infiltrated with 1% Xylocaine solution with Epinephrine to help control bleeding from stab wounds to be made at these sites. These stab wounds were made anterolaterally at the level of the superior pole of the patella for insertion of an irrigation catheter into the suprapatellar pouch area, anterolaterally at the level of the joint line for insertion of the scope and anteromedially at…
Knowledge Booster
Background pattern image
Biology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Text book image
Basic Clinical Laboratory Techniques 6E
Biology
ISBN:9781133893943
Author:ESTRIDGE
Publisher:Cengage
Text book image
Concepts of Biology
Biology
ISBN:9781938168116
Author:Samantha Fowler, Rebecca Roush, James Wise
Publisher:OpenStax College
Text book image
Biology Today and Tomorrow without Physiology (Mi...
Biology
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Text book image
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage
Text book image
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License