Microbiology: Principles and Explorations
10th Edition
ISBN: 9781119390114
Author: Black
Publisher: WILEY
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Chapter 7, Problem 1.3SC
Summary Introduction
To calculate: The average number of bacteria/ml present in the broth that is diluted to 1:1000 dilution that initially contains 37,000 bacteria/ml.
Introduction: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it contains the rigid cell wall. It is classified into different types based on the structure namely coccus (spherical shaped), Bacillus (rod-shaped), filamentous, spirilla (spiral-shaped), appendaged and pleomorphic bacteria. The structure of the bacteria can be viewed under the microscope by using the staining methods.
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What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2?
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You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria. Show your work for both.
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria.
Chapter 7 Solutions
Microbiology: Principles and Explorations
Ch. 7 - What are the differences between the lag phase and...Ch. 7 - How does logarithmic rate of increase differ from...Ch. 7 - Prob. 1.3SCCh. 7 - Why does a direct microscopic count of bacteria...Ch. 7 - What does the ending -phile mean? Distinguish...Ch. 7 - What enzymes do most obligate anaerobes lack? How...Ch. 7 - Prob. 2.3SCCh. 7 - Prob. 3.1SCCh. 7 - Distinguish between the various kinds of media:...Ch. 7 - What is the purpose of a stock culture? Why is it...
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- Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration.arrow_forwardWith a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?arrow_forwardYou have found that the D-value (decimal reduction value) of an antimicrobial agent to be 4 minutes when the agent was exposed to a bacterial culture having an initial total viable cel1 count of 10° CFU/mL. After 4 minutes upon addition of the antimicrobial agent, what would have been the total viable count of the culture in this experiment? A) O 10° CFU/mL B) O 10$ CFU/mL C) O 10° CFU/mL D) O 10' CFU/mLarrow_forward
- one lm of milk was added to 9 ml of sterile saline. 0.1ml of the diluted sample was put into a petri dish with melted agar. How many cfu/ml are in the milk if 200 colonies were counted?arrow_forwardA bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3 and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?arrow_forwardWhat is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 x 106 cells/mL?arrow_forward
- An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?arrow_forwardDesign a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration. show solutionarrow_forwardYou are testing a river water sample. Your "original" plate shows 100 coliform colonies. Assuming you did the serial dilution correctly, how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.arrow_forward
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