Microbiology: Principles and Explorations
10th Edition
ISBN: 9781119390114
Author: Black
Publisher: WILEY
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Chapter 7, Problem 1.3SC
Summary Introduction
To calculate: The average number of bacteria/ml present in the broth that is diluted to 1:1000 dilution that initially contains 37,000 bacteria/ml.
Introduction: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it contains the rigid cell wall. It is classified into different types based on the structure namely coccus (spherical shaped), Bacillus (rod-shaped), filamentous, spirilla (spiral-shaped), appendaged and pleomorphic bacteria. The structure of the bacteria can be viewed under the microscope by using the staining methods.
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After you feel comfortable with your counting method and identifying cells in the various stages of mitosis, use the four images below of whitefish blastula to count the cells in each stage until you reach 100 total cells, recording your data below in Data Table 1. (You may not need to use all four images. Stop counting when you reach 100 total cells.)
After totaling the cells in each stage, calculate the percent of cells in each stage. (Divide total of stage by overall total of 100 and then multiply by 100 to obtain percentage.)
Data Table 1Stage Totals PercentInterphase Mitosis: Prophase Metaphase Anaphase Telophase Cytokinesis Totals 100 100%
To find the length of time whitefish blastula cells spend in each stage, multiply the percent (recorded as a decimal, in other words take the percent number and divide by 100) by 24 hours. (Example: If percent is 20%, then Time in Hours = .2 * 24 = 4.8) Record your data in Data…
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Chapter 7 Solutions
Microbiology: Principles and Explorations
Ch. 7 - What are the differences between the lag phase and...Ch. 7 - How does logarithmic rate of increase differ from...Ch. 7 - Prob. 1.3SCCh. 7 - Why does a direct microscopic count of bacteria...Ch. 7 - What does the ending -phile mean? Distinguish...Ch. 7 - What enzymes do most obligate anaerobes lack? How...Ch. 7 - Prob. 2.3SCCh. 7 - Prob. 3.1SCCh. 7 - Distinguish between the various kinds of media:...Ch. 7 - What is the purpose of a stock culture? Why is it...
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