BIO 1408/09 PKG W/LS CODE
5th Edition
ISBN: 9781260370829
Author: Mader
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 6.2, Problem 3A
When electrons in the reaction center of PS 
are passed to an energy-acceptor molecule, they are replaced by electrons that have been given up by
a. oxygen.
b. glucose.
c. carbon dioxide.
d. water.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 6 Solutions
BIO 1408/09 PKG W/LS CODE
Ch. 6.1 - Describe the function of the chloroplast in...Ch. 6.1 - Prob. 2LOCh. 6.1 - Prob. 3LOCh. 6.1 - Prob. 1CYPCh. 6.1 - Prob. 2CYPCh. 6.1 - Distinguish between a reduction and an oxidation...Ch. 6.1 - The raw materials for photosynthesis are a. oxygen...Ch. 6.1 - Prob. 2ACh. 6.2 - Prob. 1LOCh. 6.2 - Prob. 2LO
Ch. 6.2 - Prob. 3LOCh. 6.2 - Prob. 1CYPCh. 6.2 - Prob. 2CYPCh. 6.2 - Describe the path of electrons from water to NADPHCh. 6.2 - Prob. 4CYPCh. 6.2 - When electrons in the reaction center of PS are...Ch. 6.2 - Prob. 4ACh. 6.2 - Prob. 5ACh. 6.2 - Prob. 6ACh. 6.3 - Prob. 1LOCh. 6.3 - Prob. 2LOCh. 6.3 - Prob. 3LOCh. 6.3 - Prob. 1CYPCh. 6.3 - Prob. 2CYPCh. 6.3 - Prob. 3CYPCh. 6.3 - Prob. 7ACh. 6.3 - Prob. 8ACh. 6.3 - The output of the Calvin cycle that is used to...Ch. 6.4 - Prob. 1LOCh. 6.4 - Prob. 2LOCh. 6.4 - Prob. 3LOCh. 6.4 - Prob. 4LOCh. 6.4 - Identify the differences between C 3 and C 4...Ch. 6.4 - Prob. 2CYPCh. 6.4 - Prob. 3CYPCh. 6.4 - Prob. 10ACh. 6.4 - Prob. 11ACh. 6 - Prob. S1.1BYBCh. 6 - Prob. S4.4BYBCh. 6 - Prob. S5.2BYBCh. 6 - Prob. 1TCCh. 6 - Prob. 2TCCh. 6 - Prob. 3TCCh. 6 - Prob. 4TCCh. 6 - Prob. 5TC
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