Fundamentals of Biochemistry: Life at the Molecular Level
Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
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Chapter 6, Problem 33CQ
Summary Introduction

To calculate: The ratio of the volume of a hemoglobin molecule (65 kD) of the four O2 molecules that bind and the ratio of the volume of a typical office (4×4×3m) of the typical (70-Kg) office worker that occupies it.

Summary Introduction

To compare: The ratio of volume of a hemoglobin molecule (65 kD) of the four O2 molecules in equal proportions to their molecular masses in office (4×4×3m) and that the office worker has a density of 1.0 g/cm3 and check if it matches with the expected results.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?

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Fundamentals of Biochemistry: Life at the Molecular Level

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