Microbiology: An Introduction Plus Mastering Microbiology with Pearson eText -- Access Card Package (13th Edition) (What's New in Microbiology)
Microbiology: An Introduction Plus Mastering Microbiology with Pearson eText -- Access Card Package (13th Edition) (What's New in Microbiology)
13th Edition
ISBN: 9780134688640
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 6, Problem 2CAE

Heat lamps are commonly used to maintain foods at about 50°C for as long as 12 hours in cafeteria serving lines. The following experiment was conducted to determine whether this practice poses a potential health hazard.

Beef cubes were surface-inoculated with 500,000 bacterial cells and incubated at 43–53°C to establish temperature limits for bacterial growth. The following results were obtained from heterotrophic plate counts performed on beef cubes at 6 and 12 hours after inoculation:

Chapter 6, Problem 2CAE, Heat lamps are commonly used to maintain foods at about 50C for as long as 12 hours in cafeteria

Draw the growth curves for each organism. What holding temperature would you recommend? Assuming that cooking kills bacteria in foods, how could these bacteria contaminate the cooked foods? What disease does each organism cause? (Hint: See Chapter 25.)

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Two flasks of E. coli are grown in batch culture in the same medium (2% glucose and amino acids; no nitrate) and at the same temperature (378C). Culture #1 is well aerated. Culture #2 is anoxic. After 16 hours the following observations are made: ■ Culture #1 has a high cell density; the cells appear to be in stationary phase, and the glucose level in the medium is reduced to 1.2%. ■ Culture #2 has a low cell density; the cells appear to be in logarithmic phase, although their doubling time is prolonged (over 1 hour). The glucose level is reduced to 0.2%. Why does culture #2 have so little glucose remaining relative to culture #1, even though culture #2 displayed slower growth and has less biomass?
Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.

Chapter 6 Solutions

Microbiology: An Introduction Plus Mastering Microbiology with Pearson eText -- Access Card Package (13th Edition) (What's New in Microbiology)

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cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY