Chapter 6, Problem 1CA

Summary Introduction

To write:

Treponema denticolacan be simply missed in a direct stain of a scraping of gum or tooth because the cells are distorted by the staining procedure. The reason that this bacterium could be seen with negative staining.

Introduction:

Heat fixing:

After the drying of smear at the room temperature on the slide. This slide is gripped with the help of tongs or a clothespin. Then it is passed across the flame of a Bunsen burner and numerous times to heat-kill. It attaches the organism to the slide. Heat fixation usually conserves generally the morphology but not an internal structure.