Chapter 6, Problem 16TYK

Summary Introduction

To analyze:

The experimental design to elucidate the fact whether malonate is a competitive or noncompetitive inhibitor of succinate dehydrogenase enzyme.

Introduction:

Succinate dehydrogenase (SDH) is the only inner mitochondrial membrane bound enzyme of the citric acid cycle, which play an important role in the breaking down of sugars, fatty acids and amino acids into carbon dioxide and water. This enzyme contains iron-surfur centers as electron acceptors and FAD as a prosthetic group. The enzymes can be activated or inhibited by different mechanisms. Competitive inhibitors are considered as the structural analogues of the substrates that bind to the active site in place of substrate.