BIOCHEMISTRY-ACHIEVE (1 TERM)
BIOCHEMISTRY-ACHIEVE (1 TERM)
9th Edition
ISBN: 9781319402853
Author: BERG
Publisher: MAC HIGHER
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Chapter 6, Problem 12P
Interpretation Introduction

Interpretation:

The importance of the mutant form of a thermostable DNA polymerase in the molecular-evolution experiments is to be stated.

Concept introduction:

The technique that is used to amplify the fragments of the DNA template to form particular fragments of DNA in vitro is known as the polymerase chain reaction or PCR.

The process that is used to change the genetic information of any individual that exhibits the properties of life, resulting mutation is called as mutagenesis.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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