Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 52, Problem 3Q
Why isn’t a pH indicator needed in the lactose broth fermentation tubes?
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Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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- What is solid state fermentation? What are its advantages over other fermentation systems such as submerged fermentation? Explain the production of rifamycin using SSF.arrow_forwardMenaquinones is produced by Flavobacterium meningosepticum via submerged fermentation with 60% working volume in 30-L fermentation. To obtain the crude menaquinones, the fermentation medium is centrifuged at 12,000 rpm for 15 minutes. The vacuum-freeze dried particles are crushed and collected. After that the samples are treated with methanol for 20 minutes. To remove polar lipid compounds from bacterial cells, HZ816 macroporous adsorption resin column is used with methanol-dichloromethane (l:1) as mobile phase and flow rate of 0.75 mL/min. Rotary evaporation at 500 C is carried out to concentrate the eluent. After that, the concentrated sample is purified with Bio-BeadsTM S-X3 to remove components without UV absorption. To obtain the homolog purified menaquinones, RP-C18 column is used. Recrystallization is carried out to obtain pure crystalized menaquinones. Answer the following: Indicate how to obtain the sample in the methanol solvent. Three different chromatography are used in…arrow_forwardWhat is the key difference between reactions that result in the final product of fermentation to form lactic acid or alcohol? What significance do these differences have in terms of microbial technology?arrow_forward
- Is alcoholic fermentation carried out under aerobic or anaerobic conditions? Explain.arrow_forwardWhy is a GasPak anaerobic jar necessary for the culture of anaerobes on Brewer’s anaerobic agar plates but not in tubes of fluid thioglycollate medium (FTM)?arrow_forwardHow can end products of fermentation help identify a bacterium?arrow_forward
- Butanediol production leads to greater ethanol production thanin the mixed-acid fermentation of Escherichia coli. Why?arrow_forwardWhat are fermentations and why are they useful to many microorganisms?arrow_forwardWhy is the enzyme hydrogenase useful to Desulfovibrioeven when it is not grown on H2 as electron donor?arrow_forward
- Why bacterium Thermus thermophilus is used ?arrow_forwardWhy do boiled and unboiled potato extracts differ in their action on hydrogen peroxide? Explain in 1-3 sentencesarrow_forwardWhy does the oxidase test must be performed from blood agar or another medium without a fermentable sugar as MacConkey agar?arrow_forward
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